Secretion by Saccharomyces cerevisiae of Human Apolipoprotein E as a Fusion to Serum Albumin
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概要
- 論文の詳細を見る
For secretion of human apolipoprotein E (hApoE) by Saccharomyces cerevisiae, the hApoE gene was fused to truncated human serum albumin (HSA)-encoding sequences and expressed under the control of the GAL7 promoter. When the mature region of the hApoE gene was fused to the HSA-encoding sequence without its pro-region and expressed in galactose-containing medium, the HSA-hApoE fusion protein was efficiently secreted into the medium at a maximum yield of 6.3 mg per liter.
- 社団法人日本農芸化学会の論文
- 1995-03-23
著者
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HORINOUCHI Sueharu
Department of Biotechnology, University of Tokyo
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Nomura Nobuhiko
Department Of Biotechnology The University Of Tokyo
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BEPPU Teruhiko
Department of Biotechnology, Graduate School of Agriculture and Life Sciences, The University of Tok
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Horinouchi Sueharu
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Horinouchi Sueharu
Dep. Of Biotechnology Graduate School Of Agriculture And Life Sciences Univ. Of Tokyo
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Beppu T
Department Of Applied Biological Science Nihon University
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Matsusara Nobuyuki
Research Laboratories, Toyama Chemical Co.
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Matsubara N
Snow Brand Milk Products Co. Ltd. Saitama Jpn
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Beppu Teruhiko
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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