Molecular Cloning of Thermus flavus Malate Dehydrogenase Gene
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概要
- 論文の詳細を見る
A 3.0-kb Hind III fragment of Thermus flavus AT-62 DNA cloned in Escherichia coli was shown to direct the synthesis of thermophilic malate dehydrogenase. The enzyme produced by the recombinant clone was purified by a relatively simple procedure of heat treatment and affinity chromatography nearly to homogeneity. The enzyme preparation had practically the same heat stability and kinetic and immunological properties as those of the enzyme prepared from T. flavus cells.
- 社団法人 日本農芸化学会の論文
著者
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Uozumi Takeshi
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Beppu Teruhiko
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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IIJIMA Shinji
Department of Agricultural Chemistry, The University of Tokyo
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