Increased Production of Cloned β-Galactosidase in Two-Stage Culture of Bacillus amyloliquefaciens

スポンサーリンク

概要

• 論文の詳細を見る

• Bacillus amyloliquefaciens harboring recombinant plasmid pHG5,which encodes B. stearothermophilus β-galactosidase, was cultured in a jar fermentor. By feeding lactose a considerable concentration of the enzyme was produced, but the cells stopped growing at on OD_<660> of about 30. On the other hand, the microorganism grew to a very high cell concentration with an OD_<660> of around 110 with glucose as a carbon source, but the enzyme specific activity was a half of the maximum value with lactose. Based on these facts, B. amyloliquefaciens was first grown using glucose, and the carbon source was then switched to lactose to induce β-galactosidase production. By this two-step culture method, both good cell growth and high enzyme productivity were obtained.

• 社団法人日本生物工学会の論文
• 1991-01-25

著者

• KOBAYASHI Takeshi

  Department of Electrical Engineering, Faculty of Engineering Science, Osaka University

• IIJIMA SHINJI

  Department of Chemical Engineering, Faculty of Engineering, Nagoya University

• Iijima Shinji

  Department Of Biotechnology School Of Engineering Naogya University

• Iijima Shinji

  Department Of Biotechnology Faculty Of Engineering Nagoya University

• Iijima Shinji

  Department Of Chemical Engineering Nagoya University

• LIN KONG

  Department of Biotechnology, Nagoya University

• Kobayashi Takeshi

  Department Of Biological Chemistry College Of Bioscience And Biotechnology Chubu University

• Lin Konghua

  Department Of Chemistry National Industrial Research Institute Of Nagoya

• Kobayashi T

  Dep. Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya Univ.

• Lin Kong

  Department Of Chemical Engineering Nagoya University

• Kobayashi T

  Univ. Tsukuba Ibaraki Jpn

• Kobayashi Takeshi

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

• KOBAYASHI TAKESHI

  Department of Biotechnology, Faculty of Engineering, Nagoya University

• IIJIMA Shinji

  Department of Agricultural Chemistry, The University of Tokyo

関連論文

• Efficient Regeneration from GUS-Transformed Ajuga Hairy Root
• Efficient Culture Method for Production of Plantlets from Mechanically Cut Horseradish Hairy Roots
• Production of Plantlets for Use as Artificial Seeds from Horseradish Hairy Roots Fragmented in a Blender
• Improved Stability of Metal-Insulator-Diamond Semiconductor Interface by Employing BaF_2 Insulator Film
• Bacterial Bloodstream Infection in Neutropenic Adult Patients after Myeloablative Cord Blood Transplantation : Experience of a Single Institution in Japan
• Targeting Hyperthermia for Renal Cell Carcinoma Using Human MN Antigenspecific Magnetoliposomes
• Peptide Array-Based Interaction Assay of Solid-Bound Peptides and Anchorage-Dependant Cells and Its Effectiveness in Cell-Adhesive Peptide Design (BIOINFORMATICS)
• Hidden Markov Model-Based Prediction of Antigenic Peptides That Interact with MHC Class II Molecules
• New Thermophilic Anaerobes That Decompose Crystalline Cellulose
• A Thermophilic Anaerobe (Clostridium species) Utilizing Various Biomass-Derived Carbohydrates
• Production of Acetone and Butanol Using Immobilized Growing-cells of Clostridium acetobutylicum
• Anaerobic Cellulose Digestion and Characterization of the Cultivaton Process in Tower-Type Fermentor
• Continuous Culture of RPMI 8226 Human Cells
• Kinetic Expression for Human Cell Grwoth in a Suspension Culture System
• Monitoring and Control of Cellulolytic Anaerobe Culture by Using Gas Evolved as an Indicator
• Clinical features of adult acute leukemia with 11q23 abnormalities in Japan : a co-operative multicenter study
• Human Immunodeficiency Virus Type 1 Subtype C Exhibits Higher Transactivation Actvity of Tat than Subtypes B and E
• Expression of a Thermostable Cellulase Gene from a Thermophilic Anaerobe in Saccharomyces cerevisiae
• Production of Ethanol in Repeated-Batch Fermentation with Membrane-Type Bioreactor
• Diversity of Cellulases Produced by a Thermophilic Anaerobe Strain NA10
• Molecular Cloning of Ruminococcus albus Cellulase Gene(Biological Chemistry)
• Heat Immunotherapy Using Magnetic Nanoparticles and Dendritic Cells for T-Lymphoma(MEDICAL BIOTECHNOLOGY, Advances in Biomedical Science and Engineering)
• Screening of Cytokines to Enhance Vaccine Effects of Heat Shock Protein 70-Rich Tumor Cell Lysate(MEDICAL BIOTECHNOLOGY, Advances in Biomedical Science and Engineering)
• Complete Regression of Mouse Mammary Carcinoma with a Size Greater than 15 mm by Frequent Repeated Hyperthermia Using Magnetite Nanoparticles
• Selective hyperthermia using magnetoliposomes to target cervical lymph node metastasis in a rabbit tongue tumor model
• Proliferation and Stratification of Keratinocyte on Cultured Amniotic Epithelial Cells for Tissue Engineering(MEDICAL BIOTECHNOLOGY)
• Tumor regression by combined immunotherapy and hyperthermia using magnetic nanoparticles in an experimental subcutaneous murine melanoma
• Transglutaminase-Mediated Gelatin Matrices Incorporating Cell Adhesion Factors as a Biomaterial for Tissue Engineering
• Radiation-Inducible TNF-α Gene Expression under Stress-Inducible Promoter gadd 153 for Cancer Therapy
• Bystander-Killing Effect and Cyclic Induction of TNF-α Gene under Heat-Inducible Promoter gadd 153
• Colominic Acid Production from Escherichia coli in a Fed-Batch Culture under the Control of Ammonium Ions Using an FIA System
• Effective Lactic Acid Production by Two-Stage Extractive Fermentation
• Time-Course Data Analysis of Gene Expression Profiles Reveals purR Regulon Concerns in Organic Solvent Tolerance in Escherichia coli(Microbial Physiology and Biotechnology)
• OVERPRODUCTION OF FOREIGN GENE PRODUCT IN RECOMBINANT ESCHERICHIA COLI BY IN SITU ADSORPTION OF TRYPTOPHAN
• Optimization of the Expression of a Foreign Gene from Galactose-Inducible Promoter in a Recombinant Yeast
• Increased Production of Cloned β-Galactosidase in Two-Stage Culture of Bacillus amyloliquefaciens
• Effects of Lactate and Ammonium on the Oxygen Uptake Rate of Human Cells
• Prognostic models in patients with non-small-cell lung cancer using artificial neural networks in comparison with logistic regression
• X-Ray Photoelectron Spectroscopy Characterization of Diamond Thin Film Surfaces for Electronic Device Application
• Electrical Properties of Al/(Ba_XCa_)F_2/i-Diamond Metal-Insulator-Semiconductor Structures
• Development of Dual On-Line Analyzer and Its Application to Fed-Batch Lactic Acid Fermentation
• Adaptive On-Line Optimizing Control for Lactic Acid Fermentation
• Preparation of Tumor-Specific Magnetoliposomes and Their Application for Hyperthermia
• Modeling of Consumers' Preferences for Regular Coffee Samples and Its Application to Product Design
• Determination of the Blending Ratio of Regular Coffee Samples by Information Technology
• Multiple fuzzy neural network system for outcome prediction and classification of 220 lymphoma patients on the basis of molecular profiling
• Selection of Causal Gene Sets for Lymphoma Prognostication from Expression Profiling and Construction of Prognostic Fuzzy Neural Network Models(MEDICAL BIOTECHNOLOGY)
• Fuzzy Neural Network Applied to Gene Expression Profiling for Predicting the Prognosis of Diffuse Large B-cell Lymphoma
• Affinity Partitioning of Protein A Using a Magnetic Aqueous Two-Phase System
• Inference of Common Genetic Network Using Fuzzy Adaptive Resonance Theory Associated Matrix Method(BIOINFORMATICS)
• Artificial Neural Network Predictive Model for Allergic Disease Using Single Nucleotide Polymorphisms Data
• Determination of Operating Conditions in Activated Sludge Process Using Fuzzy Neural Network and Genetic Algorithm
• Construction of COD Simulation Model for Activated Sludge Process by Recursive Fuzzy Neural Network
• Construction of COD Simulation Model for-Acivated Sludge Process by Fuzzy Neural Network
• -84- PROGNOSTIC IMPLICATION OF RIGHT AND LEFT VENTRICULAR DYSFUNCTIONS AND EXERCISE TESTING IN THE POST-MYOCARDIAL INFARCTION PATIENT : Congestive Heart Failure : FREE COMMUNICATIONS(I) : PROCEEDINGS OF THE 51th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE C
• -1053-EFFECTS OF ENDOTHELIUM-DERIVED RELAXING FACTOR ON BLOOD PRESSURE AND CENTRAL AUTONOMIC NEVER CONTROL : AN ASSESSMENT USING L-N^G-MONO-METHYLARGININE : THE 54th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY
• -0566-THE IN VIVO MEASUREMENT OF THE MYOCARDIAL INTERSTITIAL POTASSIUM AND LACTATE CONCENTRATIONS IN THE BEATING CANINE HEART USING AN IMPROVED MICRODIALSIS TECHNIQUE
• DIPYRIDAMOLE POTENTIATES EDRF-MEDIATED INHIBITION OF PLATELET AGGREGATION : THE ROLE OF DIPYRIDAMOLE IN VIVO AS A CYCLIC GMP PHOSPHODIESTERASE INHIBITOR : Myocardial Metabolism, Biochemistry : 53 Annual Scientific Meeting, Japanese Circulation Society
• Magnetic Characterization of Magnetite Particles for MR Contrast Agents
• A Preprocessing Method for Inferring Genetic Interaction from Gene Expression Data Using Boolean Algorithm(BIOINFORMATICS)
• Prognostic Predictor with Multiple Fuzzy Neural Models Using Expression Profiles from DNA Microarray for Metastases of Breast Cancer(BIOINFORMATICS)
• Proposal of Reliability Index in Search for Reliable Solutions of Reverse Calculation Based on Fuzzy Neural Network Modeling
• Evaluation of the Alzheimer-Type Dementia by Magnetic Resonance Imaging Using Fuzzy Neural Networks
• Simple and Rapid Cell Growth Assay Using Tetrazolium Violet Coloring Method for Screening of Organic Solvent Tolerant Bacteria
• Construction of an Electroencephalogram-Based Brain-Computer Interface Using an Artificial Neural Network(Welfare Engineering)
• Analysis of Organic Solvent Tolerance in Escherichia coli Using Gene Expression Profiles from DNA Microarrays
• On-Line Control of Glucose Concentration Using An Automatic Glucose Analyzer
• -P97- USEFULNESS OF RIGHT ATRIAL FUNCTION ANALYSIS IN THE PATIENTS WITH HEART DISEASE : RI : FREE COMMUNICATIONS(I) : PROCEEDINGS OF THE 51th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY
• THE POTENTIATED THROMBOLYSIS UNDER SELECTIVE INFUSION OF AUTO PLASMIN RICH PLASMA (APRP) : PTCA, Thrombosis : PROCEEDINGS OF THE 50th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY
• THE POTENTIATED THROMBOLYSIS USING AUTOPLASMIN RICH PLASMA (APRP) UNDER SELECTIVELY BLOOD FLOW OBSTRUCTION TRANSIENTLY PRODUCED BY BALLOON-TIP CATHETER : Ischemic Heart Disease (III) : FREE COMMUNICATIONS (III) : PROCEEDINGS OF THE 49th ANNUAL SCIENTIFIC
• RIGHT AND LEFT VENTRICULAR FUNCTION DURING EXERCISE IN PATIENTS WITH CORONARY ARTERY DISEASE : Radio Isotope : 46th Annual Scientific Meeting, Japanese Circulation Society
• POTENTIATION OF TOXICITY AND POSITIVE INOTROPIC EFFECT OF OUABAIN BY FUROSEMIDE IN GUINEA PIG HEART
• The Non-invasive Evaluation of Regional Myocardial Ischemia Studied by a "Stress-injected" Thallium-201 Myocardial Imaging and a Radionuclide Left Ventriculography : Symposium on Myocardial Ischemia
• Inducible Production of Erythropoietin Using New Runaway-Type Vector by Mammalian Cell Culture in Spinner Flask
• Continuous Production of Human Erythropoietin by Immobilized Recombinant L-929 Cells
• Kinetic Study of Hybridoma Metabolism and Antibody Production in Continuous Culture Using Serum-Free Medium
• Immobilization of Animal Cells Using Photo-Crosslinkable Resin
• Effect of Dissolved Oxygen Concentration on Monoclonal Antibody Production in Hybridoma Cell Cultures
• High Cell Density Cultivation of Anchorage-Dependent Cells Using a Novel Macroporous Cellulosic Support
• Formation of Inclusion Complexes between Cyclodextrins and Aromatic Compounds under pressurized Carbon Dioxide
• Effects of Oxygen Aeration on Production of Monoclonal Antibody in Immobilized Hybridoma-Cell Bioreactor
• Purification of Recombinant α-Amylase with Immuno-Affinity Chromatography Using Monoclonal Antibody
• Effective Utilization of Horseradish and Wasabi by Treatment with Supercritical Carbon Dioxide
• Synthesis of Aspartame Precursors by Enzymatic Reaction in Supercritical Carbon Dioxide(Food & Nutrition)
• Brewing of Sake from Rice and Rice-Koji Defatted by Supercritical Carbon Dioxide Treatment
• Removal of Organic Solvent from Antibiotics with Supercritical Carbon Dioxide
• Effect of Treatment with Supercritical Carbon Dioxide on Enzymatic Activity(Food & Nutrition)
• Sterilization of Microorganisms with Supercritical Carbon Dioxide(Food & Nutrition)
• Secretion of Thermophilic Bacterial Cellobiohydrolase in Saccharomyces cerevisiae
• Observation of Capacitance Hunching at the Flat-Band-Voltage in Boron-Doped Diamond Metal/Insulator/Semiconductor Structure
• Efficient MIS Electric Field Effect on Diamond Thin Film Transistors
• Electric Field Effect in Al/SrTiO_3/YBa_2Cu_3O_y Structure in the Normal State
• Dielectric Properties of SrTiO_3 Epitaxial Film and Their Application to Measurement of Work Function of YBa_2Cu_3O_y Epitaxial Film
• Analysis on Electric Field Effect in Al/SrTiO_3/YBa_2Cu_3O_y Structure
• Metal-Insulator-Superconductor Field-Effect-Transistor Using SrTiO_3/YBa_2Cu_3O_y Heteroepitaxial Films
• Epitaxial SrTiO_3 Thin Films Grown by ArF Excimer Laser Deposition
• Overproduction of Thermostable β-Galactosidase in Escherichia coli, Its Purification and Molecular Structure
• Prediction of Occurrence of Heterocapsa circularisquama Red Tide by Means of Fuzzy Neural Network
• Reduction of Pinhole Leakage Current of SrTiO_3 Films by ArF Excimer Laser Deposition with Shadow Mask ("Eclipse Method")
• Improved Surface Smoothness of YBa_2Cu_3O_y Films and Related Multilayers by ArF Excimer Laser Deposition with Shadow Mask "Eclipse Method"

もっと見る 閉じる

スポンサーリンク