High Cell Density Cultivation of Anchorage-Dependent Cells Using a Novel Macroporous Cellulosic Support

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概要

• 論文の詳細を見る

• We have developed a novel cell support made of macroporous cellulose fibers (SIC) for the culture of anchorage-dependent animal cells. We examined several anchorage-dependent cell lines and found that all of them attached to the support and grew, although the efficiency of cell adsorption to the support differed with cell line. Among the cell lines tested, simian virus 40-transformed human endothelial cell line #3 was immobilized on the support and cultured in a fluidized-bed bioreactor at a high cell density. By direct introduction of air and optimization of the medium feed rate, the cells could grow to 2×10^7 cells per cm^3-SIC and be maintained over 80 d.

• 社団法人日本生物工学会の論文
• 1992-07-25

著者

• Kamihira M

  Department Of Biotechnology School Of Engineering Nagoya University

• Kamihira Masamichi

  Nagoya University:(present Office)kyushu University

• Kamihira Masamichi

  名古屋大学 工学研究科生物機能工学

• Kamihira Masamichi

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

• KOBAYASHI Takeshi

  Department of Electrical Engineering, Faculty of Engineering Science, Osaka University

• Kamihira Masamichi

  Department Of Chemical Engineering Faculty Of Engineering Nagoya University

• OGAWA Tatsuya

  Department of Cardiovascular Surgery, Kinki University School of Medicine

• IIJIMA SHINJI

  Department of Chemical Engineering, Faculty of Engineering, Nagoya University

• Iijima Shinji

  Department Of Biotechnology School Of Engineering Naogya University

• Iijima Shinji

  Department Of Biotechnology Faculty Of Engineering Nagoya University

• TERASHIMA SHUJI

  Department of Biotechnology, Faculty of Engineering, Nagoya University

• YASUDA KIMIAKI

  Sakai Engineering Co.,

• Kobayashi Takeshi

  Department Of Biological Chemistry College Of Bioscience And Biotechnology Chubu University

• Yasuda K

  Biomaterial Co. Ltd. Fukui Jpn

• Ogawa T

  Department Of Biotechnology Faculty Of Engineering Nagoya University

• Kobayashi T

  Univ. Tsukuba Ibaraki Jpn

• Ogawa Tatsuya

  Department Of Cardiovascular Surgery Kinki University School Of Medicine

• Terashima Shuji

  Department Of Biotechnology Faculty Of Engineering Nagoya University

• Ogawa Tatsuya

  Department Of Advanced Interdisciplinary Sciences Graduate School Of Engineering Utsunomiya University

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