Host Range Specificity of a Novel Runaway-Type Vector for Mammalian Cells
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概要
- 論文の詳細を見る
For industrial applicatioins of our novel ranaway-type vector for mammalian cells based on simian virus 40,we studied host range specificity of this vector, especially with human-derived cell lines, since human cells as hosts seem to be very important for the production of pharmaceuticals by genetic engineering. The runaway-type vector showed a high replication rate at a permissive temperature 33℃ in HeLa cells and erythropoietin was produced effectively. The plasmid copy number was dependent on the temperature but was relatively low in KB cells. KB cells produced erythropoietin effectively at a non-permissive temperature of 37℃ despite the low plasmid copy number. On the other hand, neither runaway-type replication nor erythropoietin production was observed with blood cell derivatives such as HL-60 and RPMI-8226 cells.
- 社団法人日本生物工学会の論文
- 1995-04-25
著者
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Kamihira M
Department Of Biotechnology School Of Engineering Nagoya University
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Kamihira Masamichi
Nagoya University:(present Office)kyushu University
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Kamihira Masamichi
名古屋大学 工学研究科生物機能工学
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Kamihira Masamichi
Department Of Biotechnology Graduate School Of Engineering Nagoya University
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Kamihira Masamichi
Department Of Chemical Engineering Faculty Of Engineering Nagoya University
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IIJIMA SHINJI
Department of Chemical Engineering, Faculty of Engineering, Nagoya University
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Iijima Shinji
Department Of Biotechnology Faculty Of Engineering Nagoya University
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Kirinaka Hideyo
Department of Biotechnology, School of Engineering, Nagoya University
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Kurokawa Hideo
Department Of Biotechnology Faculty Of Engineering Nagoya Univeersity
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Kirinaka Hideyo
Department Of Biotechnology School Of Engineering Nagoya University
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IIJIMA Shinji
Department of Agricultural Chemistry, The University of Tokyo
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