Development of Dual On-Line Analyzer and Its Application to Fed-Batch Lactic Acid Fermentation

概要

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A novel on-line dual analyzer combined with an automatic sampling device was developed. When immobilized glucose oxidase and lactate oxidase are used in the analyzer, glucose and lactate concentration can be measured on-line, Lactate concentration did not influence the measurement of glucose concentration and vise versa. As an example of the application of the analyzer, on-off control of the glucose concentration in fed-batch lactic acid fermentation was carried out to increase the final lactate concentration as much as possible. When the glucose concentration was conrolled at 1g/l, the final lactate concentration was 57g/l, corresponding to a 21%increase over that obtained when the glucose concentration was set at 20g/l. The experimental results show that if the glucose concentration is controlled at a low value, say about 1g/l, then the accumulation of by-products such as acetate can be repressed and the yield of lactate from glucose increases.
社団法人日本生物工学会の論文
1992-01-25