Simple and Rapid Cell Growth Assay Using Tetrazolium Violet Coloring Method for Screening of Organic Solvent Tolerant Bacteria

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概要

• 論文の詳細を見る

• Tolerance to organic solvents is an important characteristic for selecting bacteria that are useful for producing commodity chemicals. An assay method for tolerance should be easy to perform and differences in tolerance should be reproducible. We propose a cell growth assay using tetrazolium violet and image analysis for assessing tolerance. When five microorganisms including Eseherichia coli were tested, the organic solvent tolerance (OST) of microorganisms used was rapidly and qualitatively assessed and the degrees of OST coincided with those from the conventional method using solvent overlaid-solid medium. Moreover, the proposed method was applicable to the estimation of OST for cyclohexane resistant E. coil OST3410 using various organic solvent mixtures.

• 社団法人日本生物工学会の論文
• 2003-10-25

著者

• Honda Hiroyuki

  Department of Biotechnology, School of Engineering, Nagoya University

• 本多 裕之

  名大院工・生物機能

• Kobayashi T

  School Of Bioscience And Biotechnology Chubu University

• KOBAYASHI Takeshi

  Department of Electrical Engineering, Faculty of Engineering Science, Osaka University

• Kobayasi T

  School Of Bioscience And Biotechnology Chubu University

• Honda Hiroyuki

  Department Of Bioengineering Tokyo Institute Of Technology

• HAYASHI SHUHEI

  Department of Pediatrics, Fukui Red Cross Hospital

• Kobayashi T

  Graduate School Of Marine Science And Technology Tokyo University Of Marine Science And Technology

• Kobayashi Takeshi

  Department Of Biological Chemistry College Of Bioscience And Biotechnology Chubu University

• Kobayashi T

  Dep. Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya Univ.

• Hayashi S

  Department Of Biotechnology School Of Engineering Nagoya University

• Hayashi Shuhei

  Department Of Biotechnology School Of Engineering Nagoya University

• Kobayashi T

  Graduate School Of Engineering Science Osaka University

• Honda Hiroyuki

  Department Of Anesthesia Niigata City General Hospital

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