Droplet-based gene expression analysis using a device with magnetic force-based-droplet-handling system(METHODS)

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概要

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• A droplet-based cell lysis and reverse transcription-polymerase chain reaction (PCR) were performed on-chip employing magnetic force-based-droplet-handling system. The actuation with a magnet offers a simple system for droplet manipulation; it does not need mechanical fluidic systems such as pumps and valves for handling solutions. It can be used as a powerful tool for various biochemical applications by moving and coalescing sample droplets using magnetic beads immersed in mineral oil. The droplet containing magnetic beads and the cells were manipulated with the magnet located underneath the channel, and coalesced with a droplet of lysis buffer. Using K562 cells as the leukemia model, the cell lysis, cDNA synthesis, and amplification of WT1 gene that is known as the prognostic factor for acute leukemia were successfully performed from a single cell.

著者

• Honda Hiroyuki

  Department of Biotechnology, School of Engineering, Nagoya University

• 本多 裕之

  名大院・工・生物機能

• Okochi Mina

  Department of Biotechnology, School of Engineering, Nagoya University

• Tsuchiya Hiroyoshi

  Department of Biotechnology, School of Engineering, Nagoya University

• Kumazawa Fumitaka

  Department of Biotechnology, School of Engineering, Nagoya University

• Shikida Mitsuhiro

  Department of Micro-Nano Systems Engineering, Nagoya University

• Honda Hiroyuki

  Department Of Biotechnology Graduate School Of Engineering Nagoya University:mext Innovative Researc

• 本多 裕之

  Department Of Biotechnology School Of Engineering Nagoya University

• Honda Hiroyuki

  Department Of Bioengineering Tokyo Institute Of Technology

• Kumazawa Fumitaka

  Department Of Biotechnology School Of Engineering Nagoya University

• Hosokawa Hiroyuki

  Department Of Biotechnology Graduate School Of Engineering Nagoya University:mext Innovative Researc

• Shikida Mitsuhiro

  Department Of Micro-nano Systems Engineering Nagoya University

• Tsuchiya Hiroyoshi

  Department Of Biotechnology School Of Engineering Nagoya University

• Hosokawa Hiroyuki

  Department Of Immunology Graduate School Of Medicine Chiba University

• Honda Hiroyuki

  Department Of Chemical Engineering Faculty Of Engineering Nagoya University

• Hamada Hirobumi

  Department Of Molecular Medicine Sapporo Medical University School Of Medicine

• Okochi Mina

  Department Of Biotechnology Nagoya University

• Hamada Hirobumi

  Department Of Biotechnology School Of Engineering Nagoya University

• Honda Hiroyuki

  Department Of Anesthesia Niigata City General Hospital

• Okochi Mina

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

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