Magnetic manipulation device for the optimization of cell processing conditions(MEDICAL BIOTECHNOLOGY)

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概要

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• Variability in human cell phenotypes make it's advancements in optimized cell processing necessary for personalized cell therapy. Here we propose a strategy of palm-top sized device to assist physically manipulating cells for optimizing cell preparations. For the design of such a device, we combined two conventional approaches: multi-well plate formatting and magnetic cell handling using magnetite cationic liposomes (MCLs). From our previous works, we showed the labeling applications of MCL on adhesive cells for various tissue engineering approaches. To feasibly transfer cells in multi-well plate, we here evaluated the magnetic response of MCL-labeled suspension type cells. The cell handling performance of Jurkat cells proved to be faster and more robust compared to MACS (Magnetic Cell Sorting) bead methods. To further confirm our strategy, prototype palm-top sized device "magnetic manipulation device (MMD)" was designed. In the device, the actual cell transportation efficacy of Jurkat cells was satisfying. Moreover, as a model of the most distributed clinical cell processing, primary peripheral blood mononuclear cells (PBMCs) from different volunteers were evaluated. By MMD, individual PBMCs indicated to have optimum Interleukin-2 (IL-2) concentrations for the expansion. Such huge differences of individual cells indicated that MMD, our proposing efficient and self-contained support tool, could assist the feasible and cost-effective optimization of cell processing in clinical facilities.

著者

• ITO Hiroshi

  Department of Internal Medicine, Division of Cardiovascular Medicine, Akita University Faculty of Me

• Honda Hiroyuki

  Department of Biotechnology, School of Engineering, Nagoya University

• 本多 裕之

  名大院・工・生物機能

• Kato Ryuji

  Department of Biotechnology, School of Engineering, Nagoya University

• Ino Kosuke

  Graduate School of Environmental Studies, Tohoku University

• Kato Ryuji

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

• Honda Hiroyuki

  Department Of Biotechnology Graduate School Of Engineering Nagoya University:mext Innovative Researc

• Kato Ryuji

  Department Of Biotechnology School Of Engineering Nagoya University

• Honda Hiroyuki

  Department Of Bioengineering Tokyo Institute Of Technology

• Honda Hiroyuki

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

• Ino Kosuke

  Graduate School Of Environmental Studies Tohoku Univ. Aramaki 6-6-11-604 Aoba Sendai 980-8579 Jpn

• Ino Kosuke

  Graduate School Of Environmental Studies Tohoku University

• Hosokawa Hiroyuki

  Department Of Biotechnology Graduate School Of Engineering Nagoya University:mext Innovative Researc

• Honda Hiroyuki

  Department Of Cardiovascular Research R.i.e.m. Nagoya University

• Honda Hiroyuki

  Department Of Quantum Engineering Nagoya University

• Honda Hiroyuki

  Graduate School Of Engineering Nagoya University

• Hosokawa Hiroyuki

  Department Of Immunology Graduate School Of Medicine Chiba University

• Honda Hiroyuki

  Department Of Chemical Engineering Faculty Of Engineering Nagoya University

• Ito Hiroshi

  Department Of Cardiovascular And Respiratory Medicine Akita University Graduate School Of Medicine

• Kato Ryuji

  名古屋大学 工学研究科バイオテクノロジー講座

• Hamada Hirobumi

  Department Of Biotechnology School Of Engineering Nagoya University

• Honda Hiroyuki

  Department Of Anesthesia Niigata City General Hospital

• Ito Hiroshi

  Department Neurosurgery Tokyo Medical College

• Ito Hiroshi

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

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