Cytokine Reducing Effect of Azelnidipine in Human Peripheral Blood Mononuclear Cells
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概要
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Numerous clinical trials have shown that calcium channel blocker (CCB) therapy improves the clinical outcome in patients with cardiovascular diseases. Since the progression of several types of cardiovascular diseases is closely associated with inflammation, alleviation of inflammation may be one potential mechanism of those beneficial effects of CCB therapy. We examined whether a new CCB (azelnidipine) could influence the inflammatory response of human peripheral blood mononuclear cells (PBMCs), which are recruited to inflammatory lesions and modulate inflammation. We investigated whether azelnidipine affected intracellular signaling and cytokine production by phytohemagglutinin (PHA)-stimulated human PBMCs in vitro. PBMCs were obtained from 10 healthy volunteers and stimulated with PHA. Then relative intracellular calcium ion concentration ([Ca2+]i) was assessed by fluorescence microscopy, and the production of monocyte chemoattractant protein-1 (MCP-1) and tumor necrosis factor-alpha (TNF-α) were measured by enzyme-linked immunosorbent assay. Stimulation with PHA significantly raised [Ca2+]i and enhanced the production of MCP-1 and TNF-α by human PBMCs. Azelnidipine significantly diminished the PHA-induced rise of [Ca2+]i, and the production of MCP-1 and TNF-α. These findings indicate that azelnidipine might have an anti-inflammatory influence on human PBMCs, although the mechanisms and the difference from other CCBs still remain unclear and further exploration should be required.
著者
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ITO Hiroshi
Department of Internal Medicine, Division of Cardiovascular Medicine, Akita University Faculty of Me
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Ohe Tohru
Department of Cardiovascular Medicine, Okayama University Graduate School of Medical and Dentistry
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Morita Hiroshi
Department of Cardiology, Shingu Munichipal Hospital
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Nagase Satoshi
Department of Cardiovascular Medicine Okayama University
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Nakamura Kazufumi
Department of Cardiovascular Medicine Okayama University
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Kusano Kengo
Department of Cardiovascular Medicine Okayama University
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Miura Daiji
Department of Cardiovascular Medicine, Okayama University Graduate School of Medicine
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Miura Aya
Department of Cardiovascular Medicine, Okayama University
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Hisamatsu Kenichi
Department Of Cardiovascular Medicine Okayama University Graduate School Of Medicine Dentistry And P
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Nakamura K
Division Of Cardiology Gunma Prefectural Cardiovascular Center
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Nagase Satoshi
Department Of Cardiovascular Medicine Okayama University Graduated School Of Medicine Density And Ph
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Matsubara Hiromi
Division Of Cardiology National Hospital Organization Okayama Medical Center
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Nakamura Koki
Division Of Cardiology Gunma Prefectural Cardiovascular Center
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Ohe Tohru
Department Of Cardiovascular Medicine Okayama University
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Kusano Kengo
Department Of Cardiology Okayama University Graduate School Of Medicine Dentistry And Pharmaceutical
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Nakamura Kazufumi
Department Of Cardiology Okayama University Graduate School Of Medicine Dentistry And Pharmaceutical
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MIURA Ryuzea
Department of Cardiovascular Medicine, Okayama University Graduate School of Medicine, Dentistry and
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KAJIYA Masahito
Department of Cardiovascular Medicine, Okayama University Graduate School of Medicine, Dentistry and
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Hisamatsu Kenichi
Department Of Cardiology National Hospital Organization Okayama Medical Center
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Ito Hiroshi
Department Of Cardiovascular And Respiratory Medicine Akita University Graduate School Of Medicine
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Nakamura Kazufumi
Division Of Cardiology Gunma Prefectural Cardiovascular Center
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Miura Aya
Department Of Cardiovascular Medicine Okayama University Graduate School Of Medicine Dentistry And P
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Kajiya Masahito
Department Of Cardiovascular Medicine Okayama University Graduate School Of Medicine Dentistry And P
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Miura Daiji
Department Of Cardiovascular Medicine Okayama University Graduate School Of Medicine Dentistry And P
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Kumagai Naoko
Department Of Cardiovascular Medicine Okayama University Graduate School Of Medicine Dentistry And P
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Nakamura Kazufumi
Department Of Cardiovascular Medicine Okayama University Graduate School Of Medicine Dentistry And P
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Nakahawa Koji
Department Of Cardiology Okayama University
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Kajiya Masahito
Department Of Cardiology The Heart Institute Of Japan Tokyo Women's Medical University
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Morita Hiroshi
Department Of Applied Chemistry School Of Engineering Kanagawa University
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Ito Hiroshi
Department Neurosurgery Tokyo Medical College
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Miura Ryuzea
Department Of Cardiovascular Medicine Okayama University Graduate School Of Medicine Dentistry And P
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Ohe Tohru
Department Of Cardiology Okayama University
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Morita Hiroshi
Department of Cardiovascular Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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Kajiya Masahito
Department of Cardiovascular Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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Ohe Tohru
Department of Cardiovascular Medicine, The Sakakibara Heart Institute of Okayama
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Kusano Kengo
Department of Cardiovascular Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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Miura Aya
Department of Cardiovascular Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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NAKAMURA Kazufumi
Department of Applied Chemistry, Tokai University
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Nagase Satoshi
Department of Cardiovascular Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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Miura Ryuzea
Department of Cardiovascular Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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Nakamura Kazufumi
Department of Applied Chemistry, Faculty of Science and Technology, Keio University
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Nagase Satoshi
Department of Cardiology, Okayama University Graduate School of Medicine
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