Enhanced Anthocyanin Production from Grape Callus in an Air-Lift Type Bioreactor Using a Viscous Additive-Supplemented Medium

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概要

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• An N-medium containing carboxymethyl cellulose (CMC) was applied to an air-lift type bioreactor culture of grape (Vitis vinifera cv. Bailey alicant A.) callus, and anthocyanin production was investigated. Grape callus grew well at an air flow rate of 80 ml/min and anthocyanin production was significantly increased in the N-medium, reaching 17 mg/l after 7 d of culture. The anthocyanin content of the N-medium was about two times higher than that of the conventional medium without CMC. The effect of air flow rate was also investigated within the range from 40 to 160 ml/min. A twofold increase in anthocyanin content was obtained at all the air flow rates tested in the N-medium. The distribution of grape callus size obtained after 7 d of the bioreactor culture was investigated. The average callus size was 490 μm which was 1.6 times larger than that obtained in the conventional medium. It was found that large calli with a relatively high anthocyanin pigment content were formed in the bioreactor culture using the N-medium. The fluid dynamics in the bioreactor was also investigated at three points (top, middle, and bottom) in the bioreactor by laser doppler velocimetry. The average axial velocity of the circulated medium was 0.4 times lower than that of the conventional medium while their average radial velocities were almost the same (zero). The standard deviation of radial velocity fluctuation in the N-medium was also 0.4 times less than that in the conventional medium. These results suggest that turbulent flow occurred in the bioreactor culture using the conventional medium and the degree of turbulent flow decreased significantly when 0.8% CMC was added to the medium to prepare the N-medium. A change of the flow pattern is considered to be the cause of the decrease in hydrodynamic stress, resulting in enhanced pigment production due to the enlargement of the callus.

• 社団法人日本生物工学会の論文
• 2002-08-25

著者

• Honda Hiroyuki

  Department of Biotechnology, School of Engineering, Nagoya University

• Kato Yoshihito

  Department of Life and Materials Engineering, Nagoya Institute of Technology

• 本多 裕之

  名大院工・生物機能

• Kobayashi T

  School Of Bioscience And Biotechnology Chubu University

• Nagamori Eiji

  Department Of Biotechnology School Of Engineering Nagoya University

• Nagamori Eiji

  Toyota Central R & D Labs. Inc.

• Nagamori E

  Osaka Univ. Osaka Jpn

• Nagamori Eiji

  Biotechnology Laboratory Toyota Central R&d Labs Inc.

• KOBAYASHI Takeshi

  Department of Electrical Engineering, Faculty of Engineering Science, Osaka University

• Kobayasi T

  School Of Bioscience And Biotechnology Chubu University

• HIRAOKA Setsuro

  Department of Applied Chemistry, Nagoya Institute of Technology

• Nagamori Eiji

  Toyota Central R&d Labs. Inc. 41-1 Yokomichi Nagakute Aichi 480-1192 Jpn

• Honda Hiroyuki

  Department Of Bioengineering Tokyo Institute Of Technology

• Kobayashi T

  Graduate School Of Marine Science And Technology Tokyo University Of Marine Science And Technology

• Kato Yoshihito

  Department Of Communications And Systems The University Of Electro-communications(present Address):i

• Kobayashi Takeshi

  Department Of Biological Chemistry College Of Bioscience And Biotechnology Chubu University

• Kato Yoshihiro

  Department Of Life And Materials Engineering Nagoya Institute Of Technology

• HIRAOKA KOUSUKE

  Department of Biotechnology, School of Engineering, Nagoya University

• OMOTE MARIKO

  Department of Biotechnology, School of Engineering, Nagoya University

• Omote Mariko

  Department Of Biotechnology School Of Engineering Nagoya University

• Hiraoka S

  Department Of Life And Material Engineering Nagoya Institute Of Technology

• Kikuta Hirokazu

  Department Of Biotechnology School Of Engineering Nagoya University

• Honda Hiroyuki

  Department Of Anesthesia Niigata City General Hospital

• Nagamori Eiji

  Department Of Biotechnology Graduate School Of Engineering Osaka University

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