Effect of Global Transcriptional Regulators Related to Carbohydrate Metabolism on Organic Solvent Tolerance in Escherichia coli(BIOCHEMICAL ENGINEERING)
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概要
- 論文の詳細を見る
The effect of global transcriptional regulators related to carbohydrate metabolism on organic solvent tolerance(OST) in Escherichia coli has been investigated. The OSTs of the E. coll K-12 BW25113 knockout mutants acrA, acrB, cm, crp, cyaA,fnr, and mlc were investigated on the basis of colony forming efficiency on an agar plate overlaid with organic solvents. The knockout mu- tants of the cyclic AMP receptor protein (Δcrp) and adenylate cyclase (ΔcyaA) were found to increase their OSTs. However, their OSTs decreased to the level of the wild-type strain when the ΔcyaA cells were grown in the presence of exogenous cyclic AMP. These results indicate that the formation of the cAMP-Crp complex is related to OST. The microbial-adhesion-to-hydrocarbon test on the Δcrp and ΔcyaA mutants revealed that these mutants bound less abundantly to the organic solvent phase. In the Δcrp and ΔcyaA mutants, the expression levels of GadB and NuoG increased to the level of the wild type. The OSTs of ΔgadB and ΔnuoG mutants decreased, suggesting that the expressions of these proteins are involved in the increased OST in the Δcrp and ΔcyaA mutants.
- 2008-04-25
著者
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Honda Hiroyuki
Department of Biotechnology, School of Engineering, Nagoya University
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Okochi Mina
Department of Biotechnology, School of Engineering, Nagoya University
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Shimizu Kazunori
Department of Biotechnology, School of Engineering, Nagoya University
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Honda Hiroyuki
Department Of Bioengineering Tokyo Institute Of Technology
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Shimizu Kazunori
Department Of Biotechnology School Of Engineering Nagoya University
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Kurimoto Masaki
Department of Biotechnology, School of Engineering, Nagoya University
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Kurimoto Masaki
Department Of Biotechnology School Of Engineering Nagoya University
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Okochi Mina
Department Of Biotechnology Nagoya University
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Okochi Mina
Dep. Of Biotechnology Nagoya Univ.
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Honda Hiroyuki
Department Of Biotechnology School Of Engineering Nagoya University:mext Innovative Research Center
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Honda Hiroyuki
Department Of Anesthesia Niigata City General Hospital
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Okochi Mina
Department Of Biotechnology Graduate School Of Engineering Nagoya University
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