Rapid Enzyme-Linked Immunosorbent Assay with Functional Magnetite Particles

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概要

• 論文の詳細を見る

• Ultrafine magnetite particles were applied to enzyme-linked immunosorbent assay (ELISA). For the assay of mouse IgG, anti-mouse IgG antibody or protein A was immobilized on the magnetite particles. The assay strategy was based on the sandwich method. Using anti-mouse IgG-magnetite and the spectrofluorometry, the mouse IgG standard curve was linear when the IgG concentration was between 5 μg/l-5 mg/l. Using these functional magnetite particles, we could shorten the assay time to on thiery-fifth that of the conventional method.

• 社団法人日本生物工学会の論文
• 1992-02-25

著者

• Honda Hiroyuki

  Department of Biotechnology, School of Engineering, Nagoya University

• Kamihira M

  Department Of Biotechnology School Of Engineering Nagoya University

• Kamihira Masamichi

  Nagoya University:(present Office)kyushu University

• Kamihira Masamichi

  名古屋大学 工学研究科生物機能工学

• Kamihira Masamichi

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

• WANG Jin

  Department of Clinical Hematology, Second Hospital of Xi'an Jiaotong University

• KOBAYASHI Takeshi

  Department of Electrical Engineering, Faculty of Engineering Science, Osaka University

• Kamihira Masamichi

  Department Of Chemical Engineering Faculty Of Engineering Nagoya University

• SHINKAI Masashige

  Department of Biotechnology, Graduate School of Engineering, Nagoya university

• Honda Hiroyuki

  Department Of Bioengineering Tokyo Institute Of Technology

• Kobayashi Takeshi

  Department Of Biological Chemistry College Of Bioscience And Biotechnology Chubu University

• Wang J

  Department Of Physics The University Of Hong Kong

• Wang Jin

  Department Of Biotechnology Faculty Of Engineering Nagoya University

• Wang Jin

  Laboratory Of Analytical Microbiology National Center For Biomedicine Analysis Institute Of Microbio

• Iwata Masashi

  Depatment Of Chemical Engineering Faculty Of Engineering Nagoya University

• Shinkai Masashige

  Dep. Of Chemistry And Biotechnology School Of Engineering Univ. Of Tokyo Tokyo 113-8656 Jpn

• Honda Hiroyuki

  Department Of Anesthesia Niigata City General Hospital

• Wang Jin

  Department Of Biological Sience And The Mervin Bovaird Center For Studies In Molecular Biology And B

• Shinkai Masashige

  Department of Biotechnology, Faculty of Engineering, Nagoya University

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