Effcient Production of Recombinant Bovine Cathepsin C by High Cell Density Culture of Methylotrophic Yeast Candida boidinii
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概要
- 論文の詳細を見る
Bovine cathepsin C production from recombinant methylotrophic yeast, Candida boidinii, was conducted in a jar-fermentor culture of fed-batch mode. Changing the carbon source from glucose to methanol induced cathepsin C expression. After changing the carbon source, intermittent addition of methanol was effective for the production of cathepsin C. During the growth phase, the level of dissolved oxygen was kept between 40 and 80% of the saturated DO level. With this control, the lack of oxygen could be avoided and cells were grown up to ca. 50 g dry cell weight/l, approximately two fold higher than that without the DO control. However, the expression of cathepsin C in the induction phase was reduced. Half volume of the medium was replaced with the same volume of the fresh medium just before the induction. The production was improved by the medium exchange and the activity of cathepsin C in the supernatant reached over 370 U/l after 16 days of induction, corresponding to a two-fold higher value than that obtained without medium exchange.
- 社団法人 化学工学会の論文
- 2002-05-01
著者
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Honda Hiroyuki
Department of Biotechnology, School of Engineering, Nagoya University
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Kobayashi T
School Of Bioscience And Biotechnology Chubu University
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KOBAYASHI Takeshi
Department of Electrical Engineering, Faculty of Engineering Science, Osaka University
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Kobayasi T
School Of Bioscience And Biotechnology Chubu University
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Honda Hiroyuki
Department Of Bioengineering Tokyo Institute Of Technology
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Kobayashi T
Graduate School Of Marine Science And Technology Tokyo University Of Marine Science And Technology
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TSUMURA Haruhiko
Pharmaceutical Laboratory Kirin Brewery Co., Ltd
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KOMEDA Toshihiro
Central Laboratories for Key Technology, Kirin Brewery Co., Ltd.
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Kobayashi Takeshi
Department Of Biological Chemistry College Of Bioscience And Biotechnology Chubu University
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Kobayashi T
Univ. Tsukuba Ibaraki Jpn
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Kano Kenjiro
Pharmaceutical Development Laboratories Kirin Brewery Co. Ltd.
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KIKUTA HIROKAZU
Department of Biotechnology, Graduate School of Engineering, Nagoya University
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KONDO KEIJI
Central Laboratories for Key Technology, Kirin Brewery Co., Ltd.
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Komeda T
Central Laboratories For Key Technology Kirin Brewery Co. Ltd.
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Komeda Toshihiro
Central Laboratories For Key Technology Kirin Brewery Co. Ltd.
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Kondo Keiji
Central Laboratories For Frontier Technology Kirin Holdings Co. Ltd.
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Tsumura Haruhiko
Pharmaceutical Development Laboratories Kirin Brewery Co. Ltd.
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Kikuta Hirokazu
Department Of Biotechnology School Of Engineering Nagoya University
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Honda Hiroyuki
Department Of Anesthesia Niigata City General Hospital
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