Expression of a Thermostable Cellulase Gene from a Thermophilic Anaerobe in Saccharomyces cerevisiae

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概要

• 論文の詳細を見る

• A cellulase gene from a thermophilic anaerobe was recloned in the yeast Saccharomyces cerevisiae. The maximum level of the gene expression in the recombinant yeast wa 4.4 times higher than that in the Escherichia coli transformant harboring the same plasmid. Cellulase activity was observed only within the yeast cells. To compare the enzymatic properties of cellulase produced by the yeast and E. coli transformants, cellulases were purified to homogeneous state by only three purificatio steps of heat treatment, and cellulose affinity and ion exchange chromatographies. The molecular weights of the enzymes produced by the yeast and E. coli were 3.8×10^4 and 4.0×10^4,respectively by SDS-polyacrylamide gel electrophoresis. Neither of the enzymes was glycosylated. Although the molecular weights were slightly different, enzymatic properties and thermostablity were almost indistinguishable between the enzymes produced by the yeast and E. coli transformants.

• 社団法人日本生物工学会の論文
• 1990-05-25

著者

• Honda Hiroyuki

  Department of Biotechnology, School of Engineering, Nagoya University

• KOBAYASHI Takeshi

  Department of Electrical Engineering, Faculty of Engineering Science, Osaka University

• SAITO Takao

  Department of Materials Chemistry , Graduate School of Osaka University

• Taya Masahito

  Department Of Chemical Engineering Faculty Engineering Science Osaka University

• Taya Masahito

  (present Address)faculty Of Engineering Science Osaka University

• Honda Hiroyuki

  Department Of Bioengineering Tokyo Institute Of Technology

• SUZUKI TOHRU

  Department of Chemical Engineering, Faculty of Engineering, Nagoya University

• HAYASHI AKIHIRO

  Department of Chemical Engineering, Faculty of Engineering, Nagoya University

• IIJIMA SHINJI

  Department of Chemical Engineering, Faculty of Engineering, Nagoya University

• Iijima Shinji

  Department Of Biotechnology Faculty Of Engineering Nagoya University

• Kobayashi Takeshi

  Department Of Biological Chemistry College Of Bioscience And Biotechnology Chubu University

• Hayashi Akihiro

  Department Of Biotechnology Faculty Of Engineering Nagoya University

• Saito T

  Kanagawa Univ. Kanagawa Jpn

• Taya Masahito

  Department Of Chemical Engineering

• So Tomoko

  Second Department Of Surgery Faculty Of Medicine University Of Occupational And Environmental Health

• Taya Masahito

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Taya M

  (present Address)faculty Of Engineering Science Osaka University

• Saito Takao

  Department Of Gastroenterological Surgery National Kyushu Cancer Center

• Honda Hiroyuki

  Department Of Anesthesia Niigata City General Hospital

• Saito Takao

  Department Of Biochemistry And Surgery Oita Medical School

• Kobayashi Takeshi

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

• KOBAYASHI TAKESHI

  Department of Biotechnology, Faculty of Engineering, Nagoya University

• IIJIMA Shinji

  Department of Agricultural Chemistry, The University of Tokyo

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