Preparation of Tumor-Specific Magnetoliposomes and Their Application for Hyperthermia

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概要

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• Magnetoliposomes (MLs) were conjugated with an antibody fragment to give specificity to a tumor. The antibody fragment was cross-linked to N-(6-maleimidocaproyloxy)-dipalmitoyl phosphatidylethanolamine (EMC-DPPE) in liposomal membrane. The immobilization of the antibody fragment was optimal when the content of EMC-DPPE was 10-wt% and the reaction time for immobilization was 18 h. The Fab fragment-conjugating MLs (FMLs) were 2.4 times higher molar immobilization density compared with the method using the whole antibody. The targetability of the FMLs to the glioma cells, U251-SP, was then investigated. The amount of FMLs uptake reached 85 pg/cell in an in vitro experiment using plastic dishes. In an in vivo experiment using glioma-harboring mice, 260 μg of the FMLs per 1 g of tumor tissue accumulated (tumor sizes was 0.1 cm3), which corresponded to approximately 60% of the total injection. This value was 7 times higher than that of the MLs. After injection of the FMLs, mice were exposed to intracellular hyperthermia using the alternating magnetic field irradiation. The temperature of tumor tissue increased to 43°C and the growth of the tumor was found to be arrested over 2 weeks. These results indicate the FMLs could target the glioma cells in vitro and in vivo, and are efficiently applicable to the hyperthermia of tumor.

• 社団法人 化学工学会の論文

著者

• Honda Hiroyuki

  Department of Biotechnology, School of Engineering, Nagoya University

• Le Biao

  名古屋大学 工研究

• Le Biao

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

• KOBAYASHI Takeshi

  Department of Electrical Engineering, Faculty of Engineering Science, Osaka University

• Yoshida Jun

  Department Of Neurosurgery Nagoya University Graduate School Of Medicine

• Wakabayashi Toshihiko

  Department of Neurosurgery, Nagoya University Graduate School of Medicine

• SHINKAI Masashige

  Department of Biotechnology, Graduate School of Engineering, Nagoya university

• Honda Hiroyuki

  Department Of Bioengineering Tokyo Institute Of Technology

• Wakabayashi T

  Department Of Neurosurgery Nagoya Central Hospital

• Kobayashi T

  Graduate School Of Marine Science And Technology Tokyo University Of Marine Science And Technology

• Wakabayashi T

  Department Of Neurosurgery Graduate School Of Medicine Nagoya University

• KITADE Tamotsu

  Department of Biotechnology, Graduate School of Engineering, Nagoya University

• Kitade Tamotsu

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

• Kobayashi Takeshi

  Department Of Biological Chemistry College Of Bioscience And Biotechnology Chubu University

• Shinkai Masashige

  Dep. Of Chemistry And Biotechnology School Of Engineering Univ. Of Tokyo Tokyo 113-8656 Jpn

• Wakabayashi Toshihiko

  Department Of Neurosurgery Graduate School Of Medicine Nagoya University

• Yoshida Jun

  Department Of Basic Science Graduate School Of Arts And Sciences The University Of Tokyo

• Wakabayasbi Tomo

  Department Of Pathology Institute Of Medical Science University Of Tokyo

• Honda Hiroyuki

  Department Of Anesthesia Niigata City General Hospital

• Shinkai Masashige

  Department of Biotechnology, Faculty of Engineering, Nagoya University

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