Development of Chitosan-Conjugated Magnetite for Magnetic Cell Separation

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概要

• 論文の詳細を見る

• For the purpose of separating microorganisms from culture broth by magnetic force, magnetic particles were conjugated with a polymer to produce polymer-conjugated magnetite(polymer-mag). Among 4 preparation methods investigated-aminosilane coupling, glycidylsilane coupling, crosslinking, and co-precipitation-, polymer-mag prepared by co-precipitation showed the highest cell recovery and high dispersibility. When various cationic, anionic, and nonionic polymers were used to prepare polymer-mag and applied to the magnetic separation for Escherichia coli, magnetite conjugated with chitosan(chitosan-mag)gave the highest cell recovery. In addition, E.coli cells could be recovered as the precipitant only 1 min after chitosan-mag was added to a cell suspension, and a clarified supernatant was obtained. The amount of E.coli cells adsorbed to the chitosan-mag was about 1 g-dry cells/g-chitosan-mag, and cell recovery of over 90% was attained in wide pH range from 3.0 to 7.0. Of 12 microorganisms tested, 4 could be recovered with chitosan-mag at recovery levels above 90%, and the adsorbed amounts exceeded 0.5 g-dry cells/g-chitosan-mag. Differences in adsorbed amounts were considered to be mainly due to the different zeta potential of the microorganisms tested.

• 社団法人日本生物工学会の論文
• 1998-08-25

著者

• Honda Hiroyuki

  Department of Biotechnology, School of Engineering, Nagoya University

• KOBAYASHI Takeshi

  Department of Electrical Engineering, Faculty of Engineering Science, Osaka University

• SHINKAI Masashige

  Department of Biotechnology, Graduate School of Engineering, Nagoya university

• Honda Hiroyuki

  Department Of Bioengineering Tokyo Institute Of Technology

• Kobayashi T

  Graduate School Of Marine Science And Technology Tokyo University Of Marine Science And Technology

• Kobayashi Takeshi

  Department Of Biological Chemistry College Of Bioscience And Biotechnology Chubu University

• Kawabe A

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

• KAWABE ATSUSHI

  Department of Biotechnology, Graduate School of Engineering, Nagoya University

• Shinkai Masashige

  Dep. Of Chemistry And Biotechnology School Of Engineering Univ. Of Tokyo Tokyo 113-8656 Jpn

• Honda Hiroyuki

  Department Of Anesthesia Niigata City General Hospital

• Shinkai Masashige

  Department of Biotechnology, Faculty of Engineering, Nagoya University

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