Enhancement of tPA Production under Hyperoxic Conditions by BHK Cells in Serum-Free and Serum-Containing Cultures
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概要
- 論文の詳細を見る
Effects of a wide range of dissolved oxygen concentration (DO, 0.5-28 mg/l) on anchorage-dependent BHK cell growth, metabolism and tPA production were examined with both serum-containing and serum-free media. In the range of DO from 0.5 to 5 mg/l, tPA production increased with an increase in DO in both media. Cell growth was higher at 5 mg/l DO than that at 0.5 or 2 mg/l DO in serum-containing medium, but it did not very in serum-free medium in this DO range. Further investigation under hyperoxic conditions (DO>6.8 mg/l) revealed that specific rates of tPA production were enhanced by 2-fold in serum-containing and 1.7-fold in serum-free media, although cell growth depressed above 5 mg/l of DO. Slight increases in specific rates of lactate accumulation and glucose consumption were observed in both media under hyperoxic conditions. In serum-free medium, cells were found to be less tolerant to hyperoxic conditions than those in serum-containing medium. A DO shift-up with shifting time of 4 h in serum-containing medium was found to influence significantly both cell growth and tPA production.
- 社団法人日本生物工学会の論文
- 1995-06-25
著者
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Honda Hiroyuki
Department of Biotechnology, School of Engineering, Nagoya University
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WANG Jin
Department of Clinical Hematology, Second Hospital of Xi'an Jiaotong University
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KOBAYASHI Takeshi
Department of Electrical Engineering, Faculty of Engineering Science, Osaka University
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Honda Hiroyuki
Department Of Bioengineering Tokyo Institute Of Technology
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Kobayashi T
Graduate School Of Marine Science And Technology Tokyo University Of Marine Science And Technology
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Kobayashi Takeshi
Department Of Biological Chemistry College Of Bioscience And Biotechnology Chubu University
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Wang J
Department Of Physics The University Of Hong Kong
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Wang Jin
Department Of Biotechnology Faculty Of Engineering Nagoya University
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Wang Jin
Laboratory Of Analytical Microbiology National Center For Biomedicine Analysis Institute Of Microbio
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WATANABE HIDEKI
Eisai Co. Ltd., Tsukuba Research Laboratories
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Watanabe Hideki
Tsukuba Research Laboratories Eisai Co. Ltd.
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Honda Hiroyuki
Department Of Anesthesia Niigata City General Hospital
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Wang Jin
Department Of Biological Sience And The Mervin Bovaird Center For Studies In Molecular Biology And B
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