Mag-Seeding of Rat Bone Marrow Stromal Cells into Porous Hydroxyapatite Scaffolds for Bone Tissue Engineering(CELL AND TISSUE ENGINEERING)
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概要
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Bone tissue engineering has been investigated as an alternative strategy for autograft trans-plantation. In the process of tissue engineering, cell seeding into three-dimensional (3-D) scaffolds is the first step for constructing 3-D tissues. We have proposed a methodology of cell seeding into 3-D porous scaffolds using magnetic force and magnetite nanoparticles, which we term Mag-seeding. In this study, we applied this Mag-seeding technique to bone tissue engineering using bone marrow stromal cells (BMSCs) and 3-D hydroxyapatite (HA) scaffolds. BMSCs were magnetically labeled with our original magnetite cationic liposomes (MCLs) having a positive surface charge to improve adsorption to cell surface. Magnetically labeled BMSCs were seeded onto a scaffold, and a 1-T magnet was placed under the scaffold. By using Mag-seeding, the cells were successfully seeded into the internal space of scaffolds with a high cell density. The cell seeding efficiency into HA scaffolds by Mag-seeding was approximately threefold larger than that by static-seeding (conventional method, without a magnet). After a 14-d cultivation period using the osteogenic induction medium by Mag-seeding, the level of two representative osteogenic markers (alkaline phosphatase and osteocalcin) were significantly higher than those by static-seeding. These results indicated that Mag-seeding of BMSCs into HA scaffolds is an effective approach to bone tissue engineering.
- 社団法人日本生物工学会の論文
- 2007-09-25
著者
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ITO Akira
Department of Physics, The Cancer Institute, Japanese Foundation for Cancer Research
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Honda Hiroyuki
Department of Biotechnology, School of Engineering, Nagoya University
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本多 裕之
名大院・工・生物機能
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本多 裕之
名大院工・生物機能
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本多 裕之
Department Of Biotechnology Graduate School Of Engineering Nagoya University:mext Innovative Researc
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Kamihira Masamichi
Department Of Chemical Engineering Faculty Of Engineering Kyushu University
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Kamihira Masamichi
Department Of Biotechnology Graduate School Of Engineering Nagoya University
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Shimizu Kazunori
Toyota Central R & D Labs. Inc.
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本多 裕之
Department Of Biotechnology School Of Engineering Nagoya University
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Shimizu Kazunori
Toyota Central R&d Labs. Inc. 41-1 Yokomichi Nagakute Aichi 480-1192 Jpn
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Shimizu Kazunori
Department of Biotechnology, School of Engineering, Nagoya University
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Ito A
The Department Of Computer Science And Systems Engineering Faculty Of Engineering Yamaguchi Universi
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Ito Akira
Dep. Of Chemical Engineering Fac. Of Engineering Kyushu Univ. 744 Motooka Nishi-ku Fukuoka 819-0395
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Honda Hiroyuki
Department Of Bioengineering Tokyo Institute Of Technology
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Honda Hiroyuki
Department Of Biotechnology Graduate School Of Engineering Nagoya University
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Shimizu Kazunori
Department Of Biotechnology School Of Engineering Nagoya University
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Hosokawa Hiroyuki
Department Of Biotechnology Graduate School Of Engineering Nagoya University:mext Innovative Researc
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Ito Akira
Dep. Of Biochemistry And Molecular Biology Tokyo Univ. Of Pharmacy And Life Sciences School Of Pharm
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Ito Akira
Department Of Chemical Engineering Faculty Of Engineering Kyushu University
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Ito A
Kyorin Pharmaceutical Co. Ltd.
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Ito Akira
Department Of Biochemistry And Molecular Biology School Of Pharmacy Tokyo University Of Pharmacy And
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Ito Akira
Graduate School Of Engineering Kyushu University
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Ito Akira
1st Laboratory Of Soil And Fertilizer Environment Division Chugoku National Agricultural Experiment
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Honda Hiroyuki
Graduate School Of Engineering Nagoya University
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Honda Hiroyuki
Department Of Chemical Engineering Faculty Of Engineering Nagoya University
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Hamada Hirobumi
Department Of Biotechnology School Of Engineering Nagoya University
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Honda Hiroyuki
Department Of Anesthesia Niigata City General Hospital
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Ito Akira
Department Of Chemical Engineering Faculty Of Engineering Kyusyu University
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Ito Akira
Department of Chemical Engineering, Faculty of Engineering, Kyusyu University
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