Molecular Characterization of a novel gene encoding an 8-kDa subunit of Antigen B from Echinococcus granulosus genotypes 1 and 6
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概要
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Elsevier, Wulamu, Mamuti ; Yasuhito, Sako ; Jean-Mathieu Bart ; Minoru, Nakao ; Xiumin Ma ; Hao, Wen ; Akira, Ito, Parasitology International, 56(4), 2007, 313-316.Antigen B in hydatid cyst fluid of Echinococcus granulosus is a polymeric lipoprotein of 160 kDa, and is an aggregate of several different but homologous small proteins with approximately 8 kDa which are encoded by a multigene family. Four genes encoding 8-kDa-subunit monomers of the antigen B have been identified from E. granulosus. Recently, we have isolated another novel gene from Echinococcus multilocularis encoding a fifth 8-kDa-subunit of AgB (named EmAgB8/5), predominantly transcribed in the adult worm, but not in vesicles of metacestodes. In this study, we cloned and characterized two EmAgB8/5 homologue genes from E. granulosus genotypes 1 and 6 by PCR, and named as EgG1AgB8/5 and EgG6AgB8/5, respectively. The phylogenetic relationship of these genes with other genes encoding the antigen B 8-kDa-subunit monomers was also discussed.
- 2007-12-01
著者
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ITO Akira
Department of Physics, The Cancer Institute, Japanese Foundation for Cancer Research
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中尾 稔
旭川医科大学寄生虫学教室
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中尾 稔
旭川医科大学皮膚科学教室
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Sako Yasuhito
Department Of Parasitology Asahikawa Medical College
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Sako Yasuhito
Department Of Epizootiology Faculty Of Veterinary Medicine Hokkaido University
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Ito Akira
Department Of Parasitology Asahikawa Medical College
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中尾 稔
旭川医大・寄生虫学
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NAKAO Minoru
Department of Parasitology, Asahikawa Medical College
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MAMUTI Wulamu
Department of Parasitology, Asahikawa Medical College
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BART Jean-Mathieu
Department of Parasitology, Asahikawa Medical College
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MA Xiumin
Department of Parasitology, Asahikawa Medical College
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WEN Hao
Xinjiang Hydatid Clinical Research Institute
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Nakao Minoru
Department Of Parasitology Asahikawa Medical College
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Nakao Minoru
Department Of Paracitology Asahikawa Medical College
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Bart Jean-mathieu
Department Of Parasitology Asahikawa Medical College
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Nakao M
Department Of Pathological Biochemistry Kyoto Pharmaceutical University
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Ito Akira
Department Of Biochemistry And Molecular Biology School Of Pharmacy Tokyo University Of Pharmacy And
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Ma Xiumin
Department Of Parasitology Asahikawa Medical College
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Mamuti Wulamu
Department Of Parasitology Asahikawa Medical College
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Ito A
Department Of Biology Faculty Of Science Kyushu University:(present Office)department Of Chemistry F
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迫 康仁
Department of Parasitology, Asahikawa Medical College
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Mamuti W
Department of Parasitology, Asahikawa Medical College
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Bart JM
Department of Parasitology, Asahikawa Medical College
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Ma X
Department of Parasitology, Asahikawa Medical College
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Wen H
Xinjiang Hydatid Clinical Research Institute
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