Construction of Heterotypic Cell Sheets by Magnetic Force-Based 3-D Coculture of HepG2 and NIH3T3 Cells(CELL AND TISSUE ENGINEERING)

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概要

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• Heterotypic 3-D coculture is essential to mimic tissues and organs, because cell-cell interaction between various types of cells is believed to be important for the activation of cellular functions. In this study, magnetic force was applied to construct a 3-D coculture system of HepG2 and NIH3T3 cells as a model of hepatocytes and mesenchymal cells. Magnetite cationic liposomes (MCLs) were used to label target cells. NIH3T3 cells labeled with MCLs were seeded onto ultralow-attachment plates, whose surface is composed of a covalently bound hydrogel layer that is hydrophilic and neutrally charged. When a magnet was placed under the plate, cells accumulated on the bottom of the well. After a 24-h incubation period, the cells formed a multilayered cell sheet, which contained the major mesenchymal extracellular matrix (ECM) components (fibronectin and type I collagen), suggesting that the use of stromal NIH3T3 cells gave sufficient strength to cell sheets. Both NIH3T3 and HepG2 cells were labeled with MCLs, and cocultured by two methods: NIH3T3 cell sheets were constructed and HepG2 cells were subsequently seeded onto NIH3T3 cell sheets, and then allowed to form layered cell sheets by applying magnetic force; or NIH3T3 and HepG2 cells were mixed and then allowed to form mixed cell sheets by applying magnetic force. These heterotypic multilayered cell sheets were successfully constructed and an enhanced albumin secretion by HepG2 cells was observed. These results suggest that the new tissue engineering technique using magnetite nanoparticles and magnetic force, to which we refer to as magnetic forcebased tissue engineering (Mag-TE), is a promising approach to construct multilayered cell sheets consisting of heterotypic cocultured cells.

• 2007-11-25

著者

• ITO Akira

  Department of Physics, The Cancer Institute, Japanese Foundation for Cancer Research

• Kawabe Yoshinori

  Department of Chemical Engineering, Faculty of Engineering, Kyushu University

• Kamihira Masamichi

  Graduate School of Systems Life Sciences, Kyushu University

• Kamihira M

  Department Of Biotechnology School Of Engineering Nagoya University

• Kamihira Masamichi

  Nagoya University:(present Office)kyushu University

• Kamihira Masamichi

  名古屋大学 工学研究科生物機能工学

• Kamihira Masamichi

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

• Kawabe Yoshinori

  Department Of Chemical Engineering Faculty Of Engineering Kyushu University

• Kawabe Yoshinori

  Nagoya University:(present Office)kyushu University

• Kawabe Yoshinori

  九州大学 大学院システム生命科学府

• Kawabe Yoshinori

  九州大学 工学研究院化学工学

• Kamihira Masamichi

  Department Of Chemical Engineering Faculty Of Engineering Nagoya University

• Ito Akira

  Department Of Chemical Engineering Faculty Of Engineering Kyushu University

• Ito Akira

  Department Of Biochemistry And Molecular Biology School Of Pharmacy Tokyo University Of Pharmacy And

• Jitsunobu Hideaki

  Department of Chemical Engineering, Faculty of Engineering, Kyushu University

• Jitsunobu Hideaki

  Department Of Chemical Engineering Faculty Of Engineering Kyushu University

• Kawabe Yoshinori

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

• Ito Akira

  Department of Chemical Engineering, Faculty of Engineering, Kyushu University

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