Production of Anti-CD2 Chimeric Antibody by Recombinant Animal Cells(CELL AND TISSUE ENGINEERING)
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概要
- 論文の詳細を見る
Expression vectors for chimeric anti-CD2 antibody were constructed in order to clarify the importance of the expression ratio of heavy (H-) and light (L-) chains of antibody to antibody production in animal cells. The antibody genes were introduced into cells using plasmid DNA vectors or replication-defective retroviral vectors. Productivity was maximal when the expression ratio of H- and L-chains was 1:1, and decreased when the ratio was not equal. We also examined the expression of antibody using one-packed vectors in which the bicistronic expression of H- and L-chain genes was mediated by an internal ribosomal entry site (IRES) sequence derived from encephalomyocarditis virus (EMCV). The translation efficiency was unbalanced between 5'Cap- and IRES-dependent genes. Using the retroviral vectors, it was estimated that the IRES-dependent translation efficiency was 5-fold lower than the 5'Cap-dependent translation efficiency. The cells exhibiting an unbalanced expression of H- and L-chains tended to accumulate H-chain protein.
- 社団法人日本生物工学会の論文
- 2004-10-25
著者
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Kawabe Yoshinori
Department of Chemical Engineering, Faculty of Engineering, Kyushu University
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Kamihira M
Department Of Biotechnology School Of Engineering Nagoya University
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Kamihira Masamichi
Nagoya University:(present Office)kyushu University
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Kamihira Masamichi
名古屋大学 工学研究科生物機能工学
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Kamihira Masamichi
Department Of Biotechnology Graduate School Of Engineering Nagoya University
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Kawabe Yoshinori
Department Of Chemical Engineering Faculty Of Engineering Kyushu University
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Kawabe Yoshinori
Nagoya University:(present Office)kyushu University
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Kawabe Yoshinori
九州大学 工学研究院化学工学
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Kawabe Yoshinori
名古屋大学 工学研究科生物機能工学
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Kamihira Masamichi
Department Of Chemical Engineering Faculty Of Engineering Nagoya University
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Matsumoto Hiroyuki
Tokyo Institute Of Technology
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Matsumoto Hiroyuki
Department of Electrical Engineering, Toyo University
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IIJIMA SHINJI
Department of Chemical Engineering, Faculty of Engineering, Nagoya University
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Iijima S
Department Of Biotechnology Graduate School Of Engineering Nagoya University
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Iijima Shinji
Department Of Biotechnology School Of Engineering Naogya University
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Iijima Shinji
Department Of Biotechnology Faculty Of Engineering Nagoya University
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Iijima S
Department Of Biotechnology Faculty Of Engineering Nagoya University
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Iijima Shinji
Department Of Chemical Engineering Nagoya University
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Nishijima Ken-ichi
Department of Biotechnology, Graduate School of Engineering, Nagoya University
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Ono Ken-ichiro
Department Of Biotechnology Graduate School Of Engineering Nagoya University
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Nishijima Ken-ichi
Nagoya University
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Hotta Akitsu
Department of Biotechnology, Graduate School of Engineering, Nagoya University
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ITOH KANAKO
Department of Biotechnology, Graduate School of Engineering, Nagoya University
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MORSHED MAHBOOB
Department of Biotechnology, Graduate School of Engineering, Nagoya University
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Itoh Kanako
Department Of Biotechnology Graduate School Of Engineering Nagoya University
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Morshed Mahboob
Department Of Biotechnology Graduate School Of Engineering Nagoya University
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Kuga Yuko
Department Of Biotechnology Graduate School Of Engineering Nagoya University
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Hotta Akitsu
Department Of Biotechnology Graduate School Of Engineering Nagoya University
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Motono Makoto
Department Of Biotechnology Graduate School Of Engineering Nagoya University
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Matsumoto H
Tokyo Institute Of Technology Department Of Mechanical Engineering And Science
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Yoshida Kazuhiro
Department Of Biotechnology Graduate School Of Engineering Nagoya University
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Kawabe Yoshishige
Nagoya University:(present Office)kyushu University
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Nishijima Ken-ichi
Department Of Biotechnology Graduate School Of Engineering Nagoya University
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Nishijima Ken-ichi
Department Of Applied Biological Chemistry The University Of Tokyo:(present Address)department Of Bl
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Matsumoto Hiroyuki
Department Of Biochemistry And Molecular Biology University Of Oklahoma Health Sciences Center
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Kawabe Yoshinori
Department Of Biotechnology Graduate School Of Engineering Nagoya University
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Matsumoto Hiroyuki
Department Of Biochemistry And Molecular Biology The University Of Oklahoma Health Sciences Center
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Iijima Shinji
Department of Biotechnology, Graduate School of Engineering, Nagoya University
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Nishijima Kenichi
Department of Biotechnology, Graduate School of Engineering, Nagoya University
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IIJIMA Shinji
Department of Agricultural Chemistry, The University of Tokyo
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