Growth and Differentiation of Cultured Fetal Hepatocytes Isolated from Various Developmental Stages
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概要
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We examined the relationship between cell proliferation and differentiation of cultured rat fetal and newborn hepatocytes isolated from various developmental stages. The albumin production rate increased along with cell growth under in vitro culture and became maximal two days after the growth cessation. AFP was secreted by both fetal and newborn hepatocytes with growth ability. Furthermore, the responses to HGF addition in fetal hepatocyte cultures were observed in terms of growth stimulation and down-regulation of the Met receptor. We also studied the changes in RB and liver enriched transcription factors (C/EBPs) for investigating the mechanism underlying proliferation and differentiation of fetal hepatocytes. Western blot analysis of hepatocytes taken from various gestation stages of rat liver showed that the expression of RB and C/EBPβ increased as gestation stage proceeded. When RB antisense S-oligonucleotide was added to the culture medium, proliferation and AFP expression increased, while C/EBPα and albumin expressions decreased. These results indicated that the tumor suppressor gene product RB had a profound role not only in cell proliferation but also hepatocyte differentiation.
- 1999-02-23
著者
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Kamihira M
Department Of Biotechnology School Of Engineering Nagoya University
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Kamihira Masamichi
Nagoya University:(present Office)kyushu University
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Kamihira Masamichi
名古屋大学 工学研究科生物機能工学
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Kamihira Masamichi
Department Of Biotechnology Graduate School Of Engineering Nagoya University
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Kamihira Masamichi
Department Of Chemical Engineering Faculty Of Engineering Nagoya University
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IIJIMA SHINJI
Department of Chemical Engineering, Faculty of Engineering, Nagoya University
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Iijima Shinji
Department Of Biotechnology School Of Engineering Naogya University
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Iijima Shinji
Department Of Biotechnology Faculty Of Engineering Nagoya University
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HAMAMOTO RYUJI
Department of Biotechnology, Graduate School of Engineering, Nagoya University
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Hamamoto Ryuji
Department Of Biotechnology Graduate School Of Engineering Nagoya University
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