Production of Insecticidal Protein of Bacillus thuringiensis by Cultivation of Recombinant Escherichia coli

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概要

• 論文の詳細を見る

• Cultures of recombinant Escherichia coli JM103 harboring the 135 k dalton (Da) insecticidal protein (IP) gene from Bacillus thuringiensis subsp. aizawai strain IPL7 were investigated to obtain high cell density and a large amount of 135kDa IP. In test tube cultures of recombinant E. coli JM103,soybean hydrolysate was apparently effective in improving 135kDa IP production. Howver, when fed-batch culture was carried out with soybean hydrolysate, a large amount of acetate accumulated in the btorh and acetate was evidently the main inhibitor of cell growth and 135kDa IP production. In recombinatn E. coli JM101,JM105 and JM109 producing 135kDa IP, the addition of soybean hydrolysate also increased 135kDa Ip production, but it stimulated acetate accumulation as well. In order to remove acetate from the broth, cultivation with crossfrow filtration was employed. The final cell concentration of recombinant E. coli JM103 was 125 g/dm^3,and 135kDa IP was produced at a concentration of 6.6 g/dm^3.

• 社団法人日本生物工学会の論文
• 1992-09-25

著者

• KOBAYASHI Takeshi

  Department of Electrical Engineering, Faculty of Engineering Science, Osaka University

• Shimizu Masatoshi

  Departments of Cardiology, Kobe National Hospital

• KOBAYASHI TAKESHI

  (Present address) Department of Chemical Engineering, Faculty of Engineering, Nagoya University

• IIJIMA SHINJI

  Department of Chemical Engineering, Faculty of Engineering, Nagoya University

• Shimizu Masatoshi

  Biotechnology Laboratory Takarazuka Research Center Sumitomo Chemical Co. Ltd.

• Shimizu Masatoshi

  Department Of Agricultural Chemistry Faculty Of Agriculture Niigata University

• Iijima Shinji

  Department Of Biotechnology Faculty Of Engineering Nagoya University

• Kobayashi Takeshi

  Department Of Biological Chemistry College Of Bioscience And Biotechnology Chubu University

• Shimizu M

  Kansai Univ. Suita Jpn

• Kobayashi T

  Dep. Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya Univ.

• Kobayashi T

  Univ. Tsukuba Ibaraki Jpn

• Kobayashi Takeshi

  Dept.of Chemical Engineering Nagoya University

• Shimizu Masatoshi

  Department Of Biotechnology Faculty Of Engineering Nagoya University

• Kobayashi Takeshi

  Department Of Chemical Engineering Faculty Of Engineering Nagoya University

• Kobayashi Takeshi

  Department Of Biotechnology Faculty Of Engineering Nagoya University

• Kobayashi Takeshi

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

• Kobayashi Takeshi

  Department Of Biotechnology:faculty Of Engineering Nagoya University

• KOBAYASHI TAKESHI

  Department of Chemical Engineering, Faculty of Engineering, Nagoya University

• KOBAYASHI TAKESHI

  Department of Biotechnology, Faculty of Engineering, Nagoya University

• IIJIMA Shinji

  Department of Agricultural Chemistry, The University of Tokyo

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