Immobilization of Animal Cells Using Photo-Crosslinkable Resin

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概要

• 論文の詳細を見る

• A photo-crosslinkable resin, BIX12,was selected from among various photo-crosslinkable resins for the immobilization of animal cells. BIX12 had no cytotoxic effect on the growth of hybridoma cells and the production of monoclonal antibody, although other photo-crosslinkable resins had significant inhibitory effects. Using BIX12-alginate hybrid gel particles, hybridoma cells could grow in the resins and produce monoclonal antibody. For the continuous production of monoclonal antibody, perfusion culture using a fluidized-bed bioreactor with direct air bubbling was carried out. By this cultivation, monoclonal antibody could be produced stably for more than 50 d. A high viable cell density of more than 10^7 cells/ml-gel was sttained, and the antibody productivity was improved 8.5-fold compared with conventional suspension culture using a spinner flask. Anchorage-dependent cells were also immobilized in the resin particles by three immobilization procedures. Among these procedures, porous BIX12 formed by adding gelatin powder provided good support strength and allowed the cells to grow on the surface inside of the support.

• 社団法人日本生物工学会の論文
• 1993-02-25

著者

• Kamihira M

  Department Of Biotechnology School Of Engineering Nagoya University

• Kamihira Masamichi

  Nagoya University:(present Office)kyushu University

• Kamihira Masamichi

  名古屋大学 工学研究科生物機能工学

• Kamihira Masamichi

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

• WANG Jin

  Department of Clinical Hematology, Second Hospital of Xi'an Jiaotong University

• KOBAYASHI Takeshi

  Department of Electrical Engineering, Faculty of Engineering Science, Osaka University

• Kamihira Masamichi

  Department Of Chemical Engineering Faculty Of Engineering Nagoya University

• IIJIMA SHINJI

  Department of Chemical Engineering, Faculty of Engineering, Nagoya University

• Iijima Shinji

  Department Of Biotechnology School Of Engineering Naogya University

• Iijima Shinji

  Department Of Biotechnology Faculty Of Engineering Nagoya University

• HATA MIKIKO

  Department of Biotechnology, Faculty of Engineering, Nagoya University

• Kobayashi Takeshi

  Department Of Biological Chemistry College Of Bioscience And Biotechnology Chubu University

• Wang J

  Department Of Physics The University Of Hong Kong

• Wang Jin

  Department Of Biotechnology Faculty Of Engineering Nagoya University

• Wang Jin

  Laboratory Of Analytical Microbiology National Center For Biomedicine Analysis Institute Of Microbio

• Hata Mikiko

  Department Of Biotechnology Factuly Of Engineering Nagoya University

• Wang Jin

  Department Of Biological Sience And The Mervin Bovaird Center For Studies In Molecular Biology And B

• IIJIMA Shinji

  Department of Agricultural Chemistry, The University of Tokyo

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