Enhancement of Transfection Efficiency Using Ligand-Modified Lipid Vesicles
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概要
- 論文の詳細を見る
Previously, we had developed a simple gene transfection technique for animal cells using cationic lipid vesicles ; a commercially available synthetic cationic surfactant, dimethyldioctadecyl ammonium bromide(DDAB)was used for making lipid vesicles. In the present study, the lipid vesicles for receptor-mediated gene transfer were modified with a ligand such as insulin and galactose residues to realize enhanced transfection efficiency and/or cell-specific gene transfection. The insulin-modified lipid vesicle solution mixed with the plasmid DNA(pCMVβ)was added to COS-7, NIH3T3, Hela or HepG2 cells ; the transfection efficiency was increased 3-4-fold in all the cell lines tested. Furthermore, a mixture of the galactose-modified lipid vesicles and plasmid pCMVβ was added to HepG2 or HuH-6 cells expressing asialoglycoprotein receptors, and the transfection efficiency was increased 3-4-fold in these cell lines.
- 社団法人日本生物工学会の論文
- 1998-05-25
著者
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Kamihira M
Department Of Biotechnology School Of Engineering Nagoya University
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Kamihira Masamichi
Nagoya University:(present Office)kyushu University
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Kamihira Masamichi
名古屋大学 工学研究科生物機能工学
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Kamihira Masamichi
Department Of Biotechnology Graduate School Of Engineering Nagoya University
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Kamihira Masamichi
Department Of Chemical Engineering Faculty Of Engineering Nagoya University
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IIJIMA SHINJI
Department of Chemical Engineering, Faculty of Engineering, Nagoya University
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Iijima Shinji
Department Of Biotechnology School Of Engineering Naogya University
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Iijima Shinji
Department Of Biotechnology Faculty Of Engineering Nagoya University
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YOU Jun
Department of Biotechnology, Graduate School of Engineering, Nagoya University
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You Jun
Department Of Biotechnology Graduate School Of Engineering Nagoya University
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