Chicken Ovalbumin Promoter Is Demethylated upon Expression in the Regions Specifically Involved in Estrogen-Responsiveness

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概要

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• Here we report the methylation status of the chicken ovalbumin promoter. Genomic DNA of oviduct from immature chickens and laying hens was analyzed through bisulfite genomic sequencing. In the ovalbumin control locus up to the 6 kb upstream region, CpG sites were methylated in immature chickens, except for several sites, and almost all CpGs residing in DNase I hypersensitive sites I, II, and III, but not IV, were selectively unmethylated in ovalbumin expressing chickens. Chromatin immunoprecipitation assays showed that the ovalbumin control region was associated with acetylated histone H3 but not with dimethylated histone H3 at Lys 27. These results demonstrate that DNA demethylation was restricted to short DNA regions of DNase I hypersensitive sites, especially to those which participated in estrogen-responsiveness, even when cells expressed extremely high levels of ovalbumin and these sites were associated with acetylated histones.

• 社団法人 日本農芸化学会の論文
• 2006-06-23

著者

• IIJIMA SHINJI

  Department of Chemical Engineering, Faculty of Engineering, Nagoya University

• Iijima S

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

• Iijima Shinji

  Department Of Biotechnology School Of Engineering Naogya University

• Iijima Shinji

  Department Of Biotechnology Faculty Of Engineering Nagoya University

• Iijima S

  Department Of Biotechnology Faculty Of Engineering Nagoya University

• Iijima Shinji

  Department Of Chemical Engineering Nagoya University

• Nishijima Ken-ichi

  Department of Biotechnology, Graduate School of Engineering, Nagoya University

• Nishijima Ken-ichi

  Nagoya University

• MORSHED MAHBOOB

  Department of Biotechnology, Graduate School of Engineering, Nagoya University

• Morshed Mahboob

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

• Sano Shusuke

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

• Motono Makoto

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

• NISHIMIYA Daisuke

  Department of Biotechnology, Graduate School of Engineering, Nagoya University

• ANDO Munetoshi

  Department of Biotechnology, Graduate School of Engineering, Nagoya University

• Ando Munetoshi

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

• Nishijima Ken-ichi

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

• Nishijima Ken-ichi

  Department Of Applied Biological Chemistry The University Of Tokyo:(present Address)department Of Bl

• Nishimiya Daisuke

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

• Iijima Shinji

  Department of Biotechnology, Graduate School of Engineering, Nagoya University

• Nishijima Kenichi

  Department of Biotechnology, Graduate School of Engineering, Nagoya University

• IIJIMA Shinji

  Department of Agricultural Chemistry, The University of Tokyo

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