Functional Expression of Streptococcal Galactosyltransferase in Baculovirus/Insect Cell Expression System(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)

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概要

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• The cpsIaJ gene of Streptococcus agalactiae type Ia codes for β-1, 4-galactosyltransferase. In this study, the functional expression of His-tagged CpsIaJ in a baculovirus expression system was performed, because the efficient functional expression of this enzyme in Escherichia coli had been unsuccessful. Using a partially purified enzyme preparation, we found that the enzyme had a restricted substrate specificity and that the entire structure of the substrate GlcNAcβ1-3Galβ1-4Glc was required for the activity. Furthermore, mutations in a conserved DXD motif caused the loss of the enzyme's activity.

• 社団法人日本生物工学会の論文
• 2006-04-25

著者

• 三宅 克英

  名大院・工・生物機能

• IIJIMA SHINJI

  Department of Chemical Engineering, Faculty of Engineering, Nagoya University

• Iijima S

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

• Iijima Shinji

  Department Of Biotechnology School Of Engineering Naogya University

• Iijima Shinji

  Department Of Biotechnology Faculty Of Engineering Nagoya University

• Iijima S

  Department Of Biotechnology Faculty Of Engineering Nagoya University

• Iijima Shinji

  Department Of Chemical Engineering Nagoya University

• 三宅 克英

  名古屋大学大学院工学研究科

• Murai K

  Osaka Univ. Osaka Jpn

• Koike Y

  Nagoya Univ. Nagoya Jpn

• KATAOKA YOHEI

  Department of Biotechnology Graduate School of Engineering, Nagoya University

• Koike Yoichi

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

• MIYAKE Katsuhide

  Ecotopia Science Institute, Nagoya University

• Ozeki Shingo

  Department of Biotechnology, Graduate School of Engineering, Nagoya University

• Miyake K

  Nagoya University

• Ozeki Shingo

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

• Miyake Katsuhide

  Ecotopia Institute Nagoya University

• 三宅 克英

  名大院・工・化学生物工

• 三宅 克英

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

• Ozaki Shingo

  Department of Biotechnology, Graduate School of Engineering, Nagoya University

• Iijima Shinji

  Department of Biotechnology, Graduate School of Engineering, Nagoya University

• IIJIMA Shinji

  Department of Agricultural Chemistry, The University of Tokyo

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