Production of Recombinant Tumor Necrosis Factor Receptor/Fc Fusion Protein by Genetically Manipulated Chickens(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
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概要
- 論文の詳細を見る
We previously reported the production of recombinant proteins using genetically manipulated chickens and quails. In this study, we constructed a retroviral vector encoding an expression cassette for a fusion protein of the extracellular domain of the human tumor necrosis factor(TNF) receptor 2 and Fc region of human IgG1(TNFR/Fc), which is expected as an effective drug for inflammatory diseases such as rheumatoid arthritis. The concentrated viral vector was injected into developing chicken embryos. The chickens that hatched stably produced TNFR/Fc in the serum and egg yolk for six months. It appears that the fused protein is transported and accumulated into yolk from the serum, which is mediated by the Fc receptor. The protein purified from the yolk and serum inhibited the cytotoxic activity of TNF-α toward L929 cells, indicating that the protein produced by the chickens is biologically active. These results indicate the effectiveness of the recovery of Fc-fused proteins from the yolk of genetically manipulated chickens.
- 社団法人日本生物工学会の論文
- 2008-05-25
著者
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Kawabe Yoshinori
Department of Chemical Engineering, Faculty of Engineering, Kyushu University
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Kamihira Masamichi
Graduate School of Systems Life Sciences, Kyushu University
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Kamihira M
Department Of Biotechnology School Of Engineering Nagoya University
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Kamihira Masamichi
Nagoya University:(present Office)kyushu University
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Kamihira Masamichi
名古屋大学 工学研究科生物機能工学
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Kamihira Masamichi
Department Of Biotechnology Graduate School Of Engineering Nagoya University
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Kawabe Yoshinori
Department Of Chemical Engineering Faculty Of Engineering Kyushu University
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Kawabe Yoshinori
Nagoya University:(present Office)kyushu University
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Kawabe Yoshinori
九州大学 大学院システム生命科学府
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Kawabe Yoshinori
九州大学 工学研究院化学工学
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Kawabe Yoshinori
名古屋大学 工学研究科生物機能工学
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YOSHIDA Kazuhiro
Department of Surgical Oncology, Gifu University
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Kamihira Masamichi
Department Of Chemical Engineering Faculty Of Engineering Nagoya University
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IIJIMA SHINJI
Department of Chemical Engineering, Faculty of Engineering, Nagoya University
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Iijima S
Department Of Biotechnology Graduate School Of Engineering Nagoya University
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Iijima Shinji
Department Of Biotechnology School Of Engineering Naogya University
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Iijima Shinji
Department Of Biotechnology Faculty Of Engineering Nagoya University
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Iijima S
Department Of Biotechnology Faculty Of Engineering Nagoya University
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Iijima Shinji
Department Of Chemical Engineering Nagoya University
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Motono Makoto
Department of Biotechnology, Graduate School of Engineering, Nagoya University
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Nishijima Ken-ichi
Department of Biotechnology, Graduate School of Engineering, Nagoya University
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Nishijima Ken-ichi
Nagoya University
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Kyogoku Kenji
Kaneka Corporation
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Watanabe Hiroyuki
Kaneka Corporation
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Yamashita Takashi
Kaneka Corporation
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Morshed Mahboob
Department Of Biotechnology Graduate School Of Engineering Nagoya University
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Kuga Yuko
Department Of Biotechnology Graduate School Of Engineering Nagoya University
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Motono Makoto
Department Of Biotechnology Graduate School Of Engineering Nagoya University
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Yoshida Kazuhiro
Department Of Biotechnology Graduate School Of Engineering Nagoya University
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Nishijima Ken-ichi
Department Of Biotechnology Graduate School Of Engineering Nagoya University
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Nishijima Ken-ichi
Department Of Applied Biological Chemistry The University Of Tokyo:(present Address)department Of Bl
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Motono Makoto
Department Of Biotechnology Graduate School Of Engineering Nagoya University:(present Office)departm
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Kawabe Yoshinori
Department Of Biotechnology Graduate School Of Engineering Nagoya University
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Iijima Shinji
Department of Biotechnology, Graduate School of Engineering, Nagoya University
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Nishijima Kenichi
Department of Biotechnology, Graduate School of Engineering, Nagoya University
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IIJIMA Shinji
Department of Agricultural Chemistry, The University of Tokyo
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