Growth Induction of Rat Primary Hepatocytes Using Antisense Oligonucleotides
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概要
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We examined growth control of adult and fetal hepatocytes by regulating the expression of cell-cycle-related proteins using antisense S-oligonucleotides to tumor suppressors retinoblastoma (RB) protein and p53, and cyclin-dependent kinase (CDK) inhibitors p21 and p27. The protein expression in both adult and fetal hepatocytes was significantly suppressed with the addition of corresponding antisense oligonucleotides at a concentration of 2.5 μM. For the evaluation of growth, ^3H-thymidine incorporation and DNA content were measured and the results demonstrated that all the antisense oligonucleotides had growth-promoting effects and the promoting potential was equivalent or slightly greater than that with the addition of hepatocyte growth factor (HGF) (10 ng/ml). The growth-promoting effect of the antisense oligonucleotides was enhanced by HGF in both adult and fetal hepatocyte cultures, and the effects on hepatocyte growth were also observed in a suspension culture.
- 1999-09-25
著者
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Yamada Keisuke
Department Of Neurosurgery Of The Department Of Clinical Laboratory Osaka Neurological Institute
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Kamihira M
Department Of Biotechnology School Of Engineering Nagoya University
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Kamihira Masamichi
Nagoya University:(present Office)kyushu University
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Kamihira Masamichi
名古屋大学 工学研究科生物機能工学
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Kamihira Masamichi
Department Of Biotechnology Graduate School Of Engineering Nagoya University
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Kamihira Masamichi
Department Of Chemical Engineering Faculty Of Engineering Nagoya University
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Yamada K
Department Of Biotechnology Graduate School Of Engineering Nagoya University
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IIJIMA SHINJI
Department of Chemical Engineering, Faculty of Engineering, Nagoya University
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Iijima Shinji
Department Of Biotechnology School Of Engineering Naogya University
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Iijima Shinji
Department Of Biotechnology Faculty Of Engineering Nagoya University
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HAMAMOTO RYUJI
Department of Biotechnology, Graduate School of Engineering, Nagoya University
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SEKO HIROFUMI
Department of Biotechnology, Graduate School of Engineering, Nagoya University
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KAMIMURA RYOSUKE
Department of Biotechnology, Graduate School of Engineering, Nagoya University
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MURAI KIYOHITO
Department of Biotechnology, Graduate School of Engineering, Nagoya University
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Yamada Ken-ichiro
Research Laboratory Tamon Sake Brewing Co. Ltd.
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Yamada Keisuke
Department Of Anesthesia Kanazawa University Hospital
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Seko Hirofumi
Department Of Biotechnology Graduate School Of Engineering Nagoya University
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Murai Kiyohito
Department Of Biotechnology Graduate School Of Engineering Nagoya University
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Hamamoto Ryuji
Department Of Biotechnology Graduate School Of Engineering Nagoya University
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YOSHIDA KAZUSHI
National Research Institute of Brewing
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Yoshida Kazushi
Research Laboratory Tamon Sake Brewing Co. Ltd.
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Kamimura Ryosuke
Department Of Biotechnology Graduate School Of Engineering Nagoya University
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IIJIMA Shinji
Department of Agricultural Chemistry, The University of Tokyo
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