Transformation of Lactobacillus acidophilus TK8912 by Electroporation with pULA105E Plasmid
スポンサーリンク
概要
- 論文の詳細を見る
An efficient method for the transformation of Lactobacillus acidophilus TK8912 by electroporation is presented. The plasmid vector pULA105E was constructed by joining a cryptic plasmid, pLA105,from L. acidophilus TK8912,the Escherichia coli vector pUC19,and the erythromycin resistance gene of the Streptococcus-Escherichia coli shuttle vector pVA838. Plasmid pULA105E was introduced into L. acidophilus TK1-5 at a frequency of 7.4×10^6 transformants per μg of plasmid DNA under optimized conditions. Other Lactobacillus strains, L. casei JCM 1053 and TK9008,were also transformed with pULA105E.
- 社団法人日本生物工学会の論文
- 1992-12-25
著者
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Yamada K
Department Of Biotechnology Graduate School Of Engineering Nagoya University
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Sakamoto Masaru
Research Laboratory Tamon Sake Brewing Co. Ltd.
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Oshimura Masao
Research Laboratory Tamon Sake Brewing Co. Ltd.
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Yamada Ken-ichiro
Research Laboratory Tamon Sake Brewing Co. Ltd.
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Oshimura M
Research Laboratory Tamon Sake Brewing Co. Ltd.
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YOSHIDA KAZUSHI
National Research Institute of Brewing
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Yoshida Kazushi
Research Laboratory Tamon Sake Brewing Co. Ltd.
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KANATANI KAZUO
Research Laboratory, Tamon Sake Brewing Co. Ltd.,
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TAHARA TAKATSUGU
Research Laboratory, Tamon Sake Brewing Co. Ltd.,
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MIURA HIROSUMI
Research Laboratory, Tamon Sake Brewing Co. Ltd.,
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Oshimura Masao
Reseach Laboratory Tamon Sake Brewing Co. Ltd.
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Miura Hirosumi
Research Laboratory Tamon Sake Brewing Co. Ltd.
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Kanatani K
Tamon Sake Brewing Co. Ltd. Hyogo Jpn
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Kanatani Kazuo
Research Laboratory Tamon Sake Brewing Co. Ltd.
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Tahara Takatsugu
Research Laboratory Tamon Sake Brewing Co. Ltd.
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