Purification and Some Properties of Acidocin 8912,a Novel Bacteriocin Produced by Lactobacillus acidophilus TK8912
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概要
- 論文の詳細を見る
Acidocin 8912,a bacteriocin produced by Lactobacillus acidophilus TK8912,was purified by ammonium sulfate fractionation and successive chromatographies on CM-cellulose, Sephadex G-50,Sephadex G-25,and reversed-phase HPLC on Aquapore RP-300. The purified acidocin 8912 migrated as a single band on SDS-PAGE. The molecular weight was estimated to be 5200 by SDS-PAGE, and 5400 by HPLC gel filtration on TSKgel G3000PW_<XL>. Both the amino acid composition and the N-terminal amino acid sequence analysis indicated that acidocin 8912 was a peptide composed of presumably 50 amino acids containing a Lys residue at the N-terminus. The purified acidocin 8912 showed a bactericidal effect on sensitive cells but not a bacteriolytic effect.
- 社団法人日本農芸化学会の論文
- 1992-08-23
著者
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Yamada K
Department Of Biotechnology Graduate School Of Engineering Nagoya University
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Sakamoto Masaru
Research Laboratory Tamon Sake Brewing Co. Ltd.
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Oshimura Masao
Research Laboratory Tamon Sake Brewing Co. Ltd.
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Oshimura M
Research Laboratory Tamon Sake Brewing Co. Ltd.
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YOSHIDA KAZUSHI
National Research Institute of Brewing
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Yoshida Kazushi
Research Laboratory Tamon Sake Brewing Co. Ltd.
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KANATANI KAZUO
Research Laboratory, Tamon Sake Brewing Co. Ltd.,
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TAHARA TAKATSUGU
Research Laboratory, Tamon Sake Brewing Co. Ltd.,
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MIURA HIROSUMI
Research Laboratory, Tamon Sake Brewing Co. Ltd.,
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Oshimura Masao
Reseach Laboratory Tamon Sake Brewing Co. Ltd.
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Miura Hirosumi
Research Laboratory Tamon Sake Brewing Co. Ltd.
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Kanatani K
Tamon Sake Brewing Co. Ltd. Hyogo Jpn
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Kanatani Kazuo
Research Laboratory Tamon Sake Brewing Co. Ltd.
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Tahara Takatsugu
Research Laboratory Tamon Sake Brewing Co. Ltd.
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