Isolation and Structural Analysis of the Phospho-β-Galactosidase Gene from Lactobacillus acidophilus

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概要

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• Lactobacillus acidophilus LTF42 metabolizes lactose by the phosphoenolpyruvate-dependent phosphotransferase system for lactose uptake and phospho-β-galactosidase(P-β-gal) that cleaves the lactose-6-phosphate formed. Strain LTF42 was found to carry a single 57-kb plasmid, pLA421. Since the Lac^- strain LTF42-1,a pLA421-cured derivative of LTF42,has lost P-β-gal activity, pLA421 is lkely to encode the gene for P-β-gal. A 4.8-kb SalI fragment of pLA421 containing the p-β-gal gene, designated pdg, was shotgun cloned in Escherichia coli. Further subcloning and deletion of this 4.8-kb SalI fragment allowed localization of the pbg gene on a minimal 1.9-kb ClaI-KpnI region. Analysis of the nucleotide sequence showed that the pbg gene was composed of 1,419bp (473amino acid residues)which can encode a protein with a molecular weight of 54,008. The deduced amino acid sequence of the pbg gene showed a degree of homology exceeding 60% with the P-β-gal amino acid sequences of other Gram-positive bacteria. Transformants carrying the reombinant plasmid pULA5EP containing the pbg gene on pLA421 expressed P-β-gal activities comparable to those of the parent strains.

• 1994-08-25

著者

• KANATANI KAZUO

  Research Laboratory, Tamon Sake Brewing Co. Ltd.,

• Oshimura Masao

  Reseach Laboratory Tamon Sake Brewing Co. Ltd.

• Kanatani Kazuo

  Research Laboratory Tamon Sake Brewing Co. Ltd.

関連論文

• Transformation of Lactobacillus acidophilus TK8912 by Electroporation with pULA105E Plasmid
• Inhibition of Hiochi-Bacteria by Nishin
• Purification and Some Properties of Acidocin 8912,a Novel Bacteriocin Produced by Lactobacillus acidophilus TK8912
• Plasmid-linked Galactose Utilization by Lactobacillus acidophilus TK8912
• Plasmid-associated Bacteriocin Production by and Immunity of Lactobacillus acidophilus TK8912
• Isolation and Structural Analysis of the Phospho-β-Galactosidase Gene from Lactobacillus acidophilus
• Characterization of a Small Cryptic Plasmid, pLA105,from lactobacillus acidophilus TK8912
• Plasmid-associated Bacteriocin Production by a Lactobacillus plantarum Strain
• Isolation and Partial Amino Acid Sequence of Bacteriocins Produced by Lactobacillus acidophilus

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