Integrase-Mediated Nonviral Gene Transfection with Enhanced Integration Efficiency

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概要

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• Retroviruses efficiently integrate their genome into the host chromosome. Two elements of the retrovirus genome are needed for the integration: Iong terminal repeats (LTRs) and integrase protein. We attempted to incorporate the retrovirus integration machinery in lipid vesicle-mediated gene transfection with the aim of achieving efficient stable transfection in a nonviral gene transfection system. A DNA fragment, in which a neomycin-resistant gene was flanked between partial LTR sequences derived from the Rous sarcoma virus (RSV), was constructed. This DNA fragment was transfected together with purified recombinant RSV integrase or integrase expression vectors by means of lipid vesicle-mediated gene transfection. The integrase-mediated transfection enhanced the stable transfection efficiency. The length and the end structure of the LTR sequences were important in achieving high efficiency. Under optimal conditions, the stable transfection efficiency showed a 16-fold improvement over that without integrase.

• 社団法人日本生物工学会の論文
• 1999-11-25

著者

• Kamihira M

  Department Of Biotechnology School Of Engineering Nagoya University

• Kamihira Masamichi

  Nagoya University:(present Office)kyushu University

• Kamihira Masamichi

  名古屋大学 工学研究科生物機能工学

• Kamihira Masamichi

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

• Kamihira Masamichi

  Department Of Chemical Engineering Faculty Of Engineering Nagoya University

• IIJIMA SHINJI

  Department of Chemical Engineering, Faculty of Engineering, Nagoya University

• Iijima Shinji

  Department Of Biotechnology School Of Engineering Naogya University

• Iijima Shinji

  Department Of Biotechnology Faculty Of Engineering Nagoya University

• Nishijima Ken-ichi

  Department of Biotechnology, Graduate School of Engineering, Nagoya University

• Nishijima Ken-ichi

  Nagoya University

• MIZUARAI Shinji

  Department of Biotechnology, Graduate School of Engineering, Nagoya University

• Nishijima Ken-ichi

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

• Mizuarai Shinji

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

• Nishijima Ken-ichi

  Department Of Applied Biological Chemistry The University Of Tokyo:(present Address)department Of Bl

• Nishijima Kenichi

  Department of Biotechnology, Graduate School of Engineering, Nagoya University

• IIJIMA Shinji

  Department of Agricultural Chemistry, The University of Tokyo

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