Immortalization of Human Endothelial Cells by Origin-defective Simian Virus 40 DNA(Biological Chemistry)
スポンサーリンク
概要
- 論文の詳細を見る
Human endothelial cells isolated from an umbilical cord vein were transfected with origin-defective simian virus 40 (SV40) DNA. Among several of the SV40 transfected clones isolated, cell lines SV-2 and SV-3 showed a normal endothelial cell morphology and extended life span, and could survive almost 100 generations. Just before crisis, the morphology of SV-3 changed. SV-3T cell line was isolated from this SV-3 culture, which acquired an almost infinite life span, rapid growth rate and the ability to grow in soft agar. At the same time, the SV-3T cell line lost the factor VIII-related antigen and normal endothelial cell morphology, and showed an abnormal chromosome number. Further characterization showed the ability of SV-2 and SV-3T to produce increasing amounts of tissue plasminogen activator and a similar level of a plasminogen activator inhibitor compared with normal human endothelial cells. These results indicate that the SV-3T cell line was transformed and acquired an infinite life span while still retaining a part of the differentiated endothelial cell characteristics.
- 社団法人日本農芸化学会の論文
- 1991-11-23
著者
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KOBAYASHI Takeshi
Department of Electrical Engineering, Faculty of Engineering Science, Osaka University
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IIJIMA SHINJI
Department of Chemical Engineering, Faculty of Engineering, Nagoya University
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Iijima Shinji
Department Of Biotechnology Faculty Of Engineering Nagoya University
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Kobayashi Takeshi
Department Of Biological Chemistry College Of Bioscience And Biotechnology Chubu University
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HISHIDA Tadashi
Biosciences Laboratory Yokohama Research Center, Mitsubishi Chemical Corporation
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Hishida Tadashi
Bioscience Laboratory Research Center Mitsubishi Kasei Co.
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ISHIDA Mitsuyoshi
Bioscience Laboratory, Research Center, Mitsubishi Kasei Co.
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NAKAJIMA-IIJIMA Sadayo
Bioscience Laboratory, Research Center, Mitsubishi Kasei Co.
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WATANAVBE Hideki
Department of Biotechnology, Faculty of Engineering, Nagoya University
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Ishida Mitsuyoshi
Bioscience Laboratory Research Center Mitsubishi Kasei Co.
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Kobayashi Takeshi
Department Of Biotechnology Faculty Of Engineering Nagoya University
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Kobayashi Takeshi
Department Of Biotechnology Graduate School Of Engineering Nagoya University
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Nakajima-iijima Sadayo
Bioscience Laboratory Research Center Mitsubishi Kasei Co.
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Watanavbe Hideki
Department Of Biotechnology Faculty Of Engineering Nagoya University
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KOBAYASHI TAKESHI
Department of Biotechnology, Faculty of Engineering, Nagoya University
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IIJIMA Shinji
Department of Agricultural Chemistry, The University of Tokyo
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