Continuous Production of Human Erythropoietin by Immobilized Recombinant L-929 Cells

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概要

• 論文の詳細を見る

• Recombinant L-929 cells transfected with the human erythropoietin (EPO) gene were immobilized in a macroporous cellulosic support and its derivatives in which charged groups or cell attachment factors were introduced. The immobilized cells were cultured in serum-containing and serum-free media. Comparable production of EPO was observed even in the esrum-free medium when a support modified by polyethyleneimine was used for immobilization. The cells immobilized on the supports were cultured in fluidized-bed and innerloop type air-lift bioreactors for continuous production of EPO. A high cell density of more than 2×10^7 cells/cm^3-support and high EPO productivity were achieved and maintained for 50d through the use of the innerloop type air-lift bioreactor. The productivity was 13.4-fold higher than that of conventional staitc cultures in petri-dishes.

• 社団法人日本生物工学会の論文
• 1994-01-25

著者

• Kamihira M

  Department Of Biotechnology School Of Engineering Nagoya University

• Kamihira Masamichi

  Nagoya University:(present Office)kyushu University

• Kamihira Masamichi

  名古屋大学 工学研究科生物機能工学

• Kamihira Masamichi

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

• KOBAYASHI Takeshi

  Department of Electrical Engineering, Faculty of Engineering Science, Osaka University

• Kamihira Masamichi

  Department Of Chemical Engineering Faculty Of Engineering Nagoya University

• OGAWA Tatsuya

  Department of Cardiovascular Surgery, Kinki University School of Medicine

• IIJIMA SHINJI

  Department of Chemical Engineering, Faculty of Engineering, Nagoya University

• Iijima Shinji

  Department Of Biotechnology School Of Engineering Naogya University

• Iijima Shinji

  Department Of Biotechnology Faculty Of Engineering Nagoya University

• TERASHIMA SHUJI

  Department of Biotechnology, Faculty of Engineering, Nagoya University

• OHNO MASANAO

  Department of Biotechnology, Faculty of Engineering, Nagoya University

• Kobayashi Takeshi

  Department Of Biological Chemistry College Of Bioscience And Biotechnology Chubu University

• Ohno Masanao

  Department Of Biotechnology Faculty Of Engineering Nagoya University

• Ogawa T

  Department Of Biotechnology Faculty Of Engineering Nagoya University

• Kobayashi T

  Univ. Tsukuba Ibaraki Jpn

• Ogawa Tatsuya

  Department Of Cardiovascular Surgery Kinki University School Of Medicine

• Terashima Shuji

  Department Of Biotechnology Faculty Of Engineering Nagoya University

• Ogawa Tatsuya

  Department Of Advanced Interdisciplinary Sciences Graduate School Of Engineering Utsunomiya University

• IIJIMA Shinji

  Department of Agricultural Chemistry, The University of Tokyo

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