Proliferation and Stratification of Keratinocyte on Cultured Amniotic Epithelial Cells for Tissue Engineering(MEDICAL BIOTECHNOLOGY)

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概要

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• Human amniotic epithelial cells (HAECs) are formed from amnioblasts, separated from the epiblast at about the 8th day after fertilization. Recent studies suggest that HAECs can produce various biologically active substances. In this study, the effects of cultured HAECs on keratinocytes were investigated. First of all, the effect of the medium conditioned by cultured HAECs on the proliferation of keratinocytes was examined. The conditioned medium significantly enhanced the proliferation (P<0.05). Next, the effect of co-culture with HAECs was also examined. The keratinocytes formed a stratified epithelium on day 7 after the start of co-culture. The cultured epithelium formed by the co-culture was five to six layers thick, could be detached by dispase treatment, and had sufficient strength as a sheet. These results suggest that HAECs will be a novel supplemental material for the tissue engineering of skin.

• 社団法人日本生物工学会の論文
• 2003-06-25

著者

• ITO Akira

  Department of Physics, The Cancer Institute, Japanese Foundation for Cancer Research

• Honda Hiroyuki

  Department of Biotechnology, School of Engineering, Nagoya University

• 本多 裕之

  名大院工・生物機能

• Kobayashi T

  School Of Bioscience And Biotechnology Chubu University

• Ueda Minoru

  Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine

• KOBAYASHI Takeshi

  Department of Electrical Engineering, Faculty of Engineering Science, Osaka University

• Kobayasi T

  School Of Bioscience And Biotechnology Chubu University

• SHINKAI Masashige

  Department of Biotechnology, Graduate School of Engineering, Nagoya university

• Ito A

  The Department Of Computer Science And Systems Engineering Faculty Of Engineering Yamaguchi Universi

• Ito Akira

  Dep. Of Chemical Engineering Fac. Of Engineering Kyushu Univ. 744 Motooka Nishi-ku Fukuoka 819-0395

• Ueda M

  Department Of Oral And Maxillofacial Surgery Nagoya University Graduate School Of Medicine

• Honda Hiroyuki

  Department Of Bioengineering Tokyo Institute Of Technology

• TAKIZAWA YOHEI

  Department of Biotechnology, School of Engineering, Nagoya University

• HATA KEN-ICHIRO

  Regenerative Medical Center, Nagoya University School of Medicine

• KUNO NAOHIKO

  Department of Obstetrics and Gynecology, Nagoya University School of Medicine

• ITAKURA ATSUO

  Department of Obstetrics and Gynecology, Nagoya University School of Medicine

• Itakura Atsuo

  Department Of Obstetrics And Gynecology Nagoya University Graduate School Of Medicine

• Kobayashi T

  Graduate School Of Marine Science And Technology Tokyo University Of Marine Science And Technology

• Kuno Naohiko

  Department Of Biochemistry Nagoya University School Of Medicine

• Hata Ken-ichiro

  Regenerative Medical Center Nagoya University School Of Medicine

• Ito Akira

  Dep. Of Biochemistry And Molecular Biology Tokyo Univ. Of Pharmacy And Life Sciences School Of Pharm

• Ito Akira

  Department Of Chemical Engineering Faculty Of Engineering Kyushu University

• Ito A

  Kyorin Pharmaceutical Co. Ltd.

• Ito Akira

  Department Of Biochemistry And Molecular Biology School Of Pharmacy Tokyo University Of Pharmacy And

• Ito Akira

  Graduate School Of Engineering Kyushu University

• Ito Akira

  1st Laboratory Of Soil And Fertilizer Environment Division Chugoku National Agricultural Experiment

• Takizawa Yohei

  Department Of Biotechnology School Of Engineering Nagoya University

• Ueda Minoru

  Department Of Oral And Maxillofacial Surgery Nagoya University Graduate School Of Medicine

• Kobayashi Takeshi

  Department Of Biological Chemistry College Of Bioscience And Biotechnology Chubu University

• Ueda M

  Department Of Oral Surgery Nagoya University School Of Medicine

• Ueda M

  Department Of Cardiac Surgery Nagoya University Graduate School Of Medicine

• Ueda Minoru

  Univ. Tokyo Jpn

• Shinkai Masashige

  Dep. Of Chemistry And Biotechnology School Of Engineering Univ. Of Tokyo Tokyo 113-8656 Jpn

• Ueda Minoru

  Department Of Chemistry Faculty Of Science Tohoku University

• Ueda Minoru

  Department Of Applied Chemistry Aichi Institute Of Technology

• Honda Hiroyuki

  Department Of Anesthesia Niigata City General Hospital

• Ito Akira

  Department Of Chemical Engineering Faculty Of Engineering Kyusyu University

• Ueda Minoru

  Department 1 Product Development Division 1 Motorcycle R&d Center Honda R&d Co. Ltd.

• Ito Akira

  Department of Chemical Engineering, Faculty of Engineering, Kyusyu University

• Shinkai Masashige

  Department of Biotechnology, Faculty of Engineering, Nagoya University

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