Directed evolution of angiotensin II-inhibiting peptides using a microbead display(METHODS)
スポンサーリンク
概要
- 論文の詳細を見る
Angiotensin II (ang II), an octapeptide (DRVYVHPF), can regulate blood pressure by binding specifically to its receptor, AT1. A peptide (VVIVIY) in the first transmembrane of AT1 has been found, via peptide array technology, to have an affinity for ang II. In this study, the peptide P2, which contained the VVIVIY sequence, was mutated and screened using microbead display technology that utilized emulsion PCR and cell-free protein synthesis. After one round of screening, the binding activities of collected mutants were estimated using flow cytometry and a peptide array. Two of these exhibited improved association rate constants to ang II, compared to the P2 peptide.
著者
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Kojima Takaaki
Laboratory of Molecular Biotechnology, Graduate School of Bioagricultural Sciences, Nagoya Universit
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Nakano Hideo
Laboratory of Molecular Biotechnology, Graduate School of Bioagricultural Sciences, Nagoya Universit
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Honda Hiroyuki
Department of Biotechnology, School of Engineering, Nagoya University
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本多 裕之
名大院・工・生物機能
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Okochi Mina
Department of Biotechnology, School of Engineering, Nagoya University
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Gan Rui
Laboratory of Molecular Biotechnology, Graduate School of Bioagricultural Sciences, Nagoya Universit
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Furuzawa Seiji
Laboratory of Molecular Biotechnology, Graduate School of Bioagricultural Sciences, Nagoya Universit
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Kanie Kei
Department of Biotechnology, School of Engineering, Nagoya University
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Kato Ryuji
Department of Biotechnology, School of Engineering, Nagoya University
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本多 裕之
名大院工・生物機能
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Kato Ryuji
Department Of Biotechnology Graduate School Of Engineering Nagoya University
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中野 秀雄
名古屋大学大学院生命農学研究科
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本多 裕之
Department Of Biotechnology Graduate School Of Engineering Nagoya University:mext Innovative Researc
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Kato Ryuji
Department Of Biotechnology School Of Engineering Nagoya University
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本多 裕之
Department Of Biotechnology School Of Engineering Nagoya University
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Gan Rui
Laboratory Of Molecular Biotechnology Graduate School Of Bioagricultural Sciences Nagoya University
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Kanie Kei
Department Of Biotechnology School Of Engineering Nagoya University
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Furuzawa Seiji
Laboratory Of Molecular Biotechnology Graduate School Of Bioagricultural Sciences Nagoya University
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Honda Hiroyuki
Department Of Bioengineering Tokyo Institute Of Technology
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Honda Hiroyuki
Department Of Biotechnology Graduate School Of Engineering Nagoya University
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Okochi Mina
Department Of Biotechnology School Of Engineering Nagoya University
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Hosokawa Hiroyuki
Department Of Biotechnology Graduate School Of Engineering Nagoya University:mext Innovative Researc
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Honda Hiroyuki
Department Of Quantum Engineering Nagoya University
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Honda Hiroyuki
Graduate School Of Engineering Nagoya University
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Hosokawa Hiroyuki
Department Of Immunology Graduate School Of Medicine Chiba University
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Honda Hiroyuki
Department Of Chemical Engineering Faculty Of Engineering Nagoya University
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Kato Ryuji
名古屋大学 工学研究科バイオテクノロジー講座
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Nakano Hideo
Laboratory Of Molecular Biotechnology Graduate School Of Bioagricultural Sciences Nagoya University
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Hamada Hirobumi
Department Of Molecular Medicine Sapporo Medical University School Of Medicine
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Kojima T
Laboratory Of Molecular Biotechnology Graduate School Of Bioagricultural Sciences Nagoya University
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Okochi Mina
Department Of Biotechnology Nagoya University
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Kojima Takaaki
Laboratory Of Chemical Biology Department Of Biological Science Graduate School Of Science Osaka Pre
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Hamada Hirobumi
Department Of Biotechnology School Of Engineering Nagoya University
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Nakano Hideo
Laboratory Of Molecular Biotechnology Department Of Bioengineering Sciences Graduate School Of Bioag
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Honda Hiroyuki
Department Of Anesthesia Niigata City General Hospital
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Nakano Hideo
Department Of Bioengineering Sciences Graduate School Of Bioagricultural Sciences Nagoya University
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Kojima Takaaki
Department Of Bioengineering Sciences Graduate School Of Bioagricultural Sciences Nagoya University
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Okochi Mina
Department Of Biotechnology Graduate School Of Engineering Nagoya University
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Gan Rui
Laboratory of Molecular Biotechnology, Graduate School of Bioagricultural Sciences, Nagoya University
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