Extracellular Production of Phospholipase D of Streptomyces antibioticus Using Recombinat Escherichia coli

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概要

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• The phospholipase D (PLD)-encoding gene of Streptomyces antibioticus fused with the pectate lyase B (PelB) signal sequence was expressed in recombinant Escherichia coli under the control of the T7 lac promoter. Most of the PLD activity wa detected in the culture supernatant.The N-terminal amino acid sequence of the recombinant PLD was indentical to that of the wild-type PLD, suggesting that the processing of the PelB signal peptide occurred correctly and that the PLD produced by E. coli was identical to the wild-type enzyme. The production of PLD was improved by genetic and fermentation techniques. About 3 mg of PLD/l medium was obtained under optimal conditions, comparable to the amount of PLD produced by S. antibioticus.The results of analysis of the distributions of intracellular marker enzymes suggested that neither cell lysis nor periplasmic disruption was the cause of the secretion of PLD.

• 社団法人日本生物工学会の論文
• 1995-05-25

著者

• Nakano Hideo

  Laboratory of Molecular Biotechnology, Graduate School of Bioagricultural Sciences, Nagoya Universit

• YAMANE TSUNEO

  Laboratory of Molecular Biotechnology, Division of Molecular and Cellular Mechanisms, graduate Schoo

• Iwasaki Yugo

  Laboratory of Molecular Biotechnology, Department of Bioengineering Sciences, Graduate School of Bio

• Iwasaki Y

  Lab. Of Molecular Biotechnology Dep. Of Bioengineering Sciences Graduate School Of Bioagricultural S

• Iwasaki Yugo

  Laboratory Of Molecular Biotechnology Department Of Bioengineering Sciences Graduate School Of Bioag

• Iwasaki Yugo

  Laboratory Of Molecular Biotechnology Graduate School Of Bio- And Agro-sciences Nagoya University

• Iwasaki Yugo

  Department Of Applied Biological Sciences Faculty Of Agriculture Nagoya University

• Yamane T

  Laboratory Of Molecular Biotechnology Graduate School Of Bioagricultural Sciences Nagoya University:

• Yamane Tsuneo

  Laboraotory Of Bioreaction Engineering Department Of Food Science And Technology Faculty Of Agricult

• Yamane Tsuneo

  Laboratory Of Bioreaction Engineering Department Of Food Science And Technology School Of Agricultur

• Yamane T

  Laboratory Of Molecular Biotechnology Graduate School Of Biological & Agricultural Sciences Nago

• Yamane T

  Laboratory Of Bioreaction Engineering Department Of Food Science And Technology School Of Agricultur

• Nakano Hideo

  Laboratory Of Molecular Biotechnology Graduate School Of Bioagricultural Sciences Nagoya University

• Yabuki Takashi

  Protein Research Group Riken Genomic Sciences Center

• MISHIMA NAOTO

  Laboratory of Molecular Biotechnology, Department of Applied Biological Science, School of Agricultu

• MIZUMOTO KOUSAKU

  Laboratory of Molecular Biotechnology, Department of Applied Biological Sciences, School of Agricult

• Mizumoto Kousaku

  Laboratory Of Molecular Biotechnology Department Of Applied Biological Sciences School Of Agricultur

• Mishima Naoto

  Laboratory Of Molecular Biotechnology Department Of Applied Biological Science School Of Agriculture

• Yamane Tsuneo

  Dept. Of Food Science And Technology Faculty Of Agriculture Nagoya University

• Yamane Tsuneo

  Laboratory Of Molecular Biotechnology Graduate School Of Bio- And Agro-sciences Nagoya University

• Nakano Hideo

  Laboratory Of Molecular Biotechnology Department Of Bioengineering Sciences Graduate School Of Bioag

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