Some Characteristics of Continuous Glyceride Synthesis by Lipase in a Microporous Hydrophobic Membrane Bioreactor

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概要

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• Continuous synthesis of oleic acid glycerides by seven Upases available commercially were compared at 40°C using the microporous hydrophobic membrane bioreactor we developed recently. The lipases produced by Rhizopus japonicus, Candida cylindracea, and Phycomyces nitens were quickly inactivated in 97% glycerol solution and yielded very low glyceride conversions. The other four lipases yielded glyceride synthesis activities in the order: Rhizopus delemers<Pseudomonas fluorescenss< Mucor mieheis<Chromobacterium viscosums. For these four lipases, there was a hyperbola-like relationship between the conversion and the amount of lipase adsorbed at the membrane during the reaction. The adsorbed lipase desorbed relatively easily when glycerol-water-lipase solution was replaced by fresh 97% glycerol solution having no lipases. Mucor mieheis lipase was adsorbed more strongly than Chromobacterium viscosums. The product produced by Mucor mieheis lipase was composed mostly of mono- and di-glycerides while the one produced by Pseudomonas fluorescenss was composed of mono-, di-, and tri-glycerides with an approximate molar ratio of 3:4:1. The contents of 2-monoglyceride and 1, 2-diglyceride isomers in the product having about 80% conversion produced by Mucor mieheis lipase were quite small. The approximate half-life of Mucor mieheis lipase was estimated as 54 days from a continuous reaction experiment carried out for 27 days.

• 社団法人 日本農芸化学会の論文

著者

• Yamane Tsuneo

  Laboraotory Of Bioreaction Engineering Department Of Food Science And Technology Faculty Of Agricult

• Shimizu Shoichi

  Laboraotory Of Bioreaction Engineering Department Of Food Science And Technology Faculty Of Agricult

• TAGAMI Haruko

  Laboratory of Bioreaction Engineering, Department of Food Science and Technology, Faculty of Agriculture, Nagoya University

• HOQ Mohammad

  Laboratory of Bioreaction Engineering, Department of Food Science and Technology, Faculty of Agriculture, Nagoya University

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