Detection and Automatic Control of Ammonium Ion Concentration in Microbial Culture with an Ammonium Ion Selective Electrode
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概要
- 論文の詳細を見る
An ammonium ion selective electrode (AISE) had a membrane of polyvinyl chloride in which the antibiotics nonactin and monactin were embedded. The detection range was 0.1-200 mM. The step response was 90% in 20 seconds. The output of the AISE increased 6% with a 1℃ rise of temperature. The output of the AISE was constant between pH 4-7. The selectivity coefficient of potassium ion was 0.158 and hence its interfering effect must be considered. The selectivity coefficients of other cations were small enough to be negligible. Throughout a batch culture of Escherichia coli, values calculated by subtracting (selectivity coefficient)×(potassium ion concentration) from the detected output of the AISE agreed with actual concentrations of ammonium ion. An automatic, constant-value, feedback control system of ammonium ion concentration was attempted by on-off controlled supply of solution containing both ammonium and potassium ions, the proportion of whose concentrations was made equal to the proportion of their average volumetric consumption rates by a microorganism in batch culture. By this control system, ammonium ion concentrations in culture super-natants of fed-batch cultures of Escherichia coli and Saccharomyces cerevisiae could be maintained virtually at constant levels (5±0.8 mM for the cultivation of E. coli and 50±5 mM for the cultivation of S. cerevisiae).
- 社団法人日本生物工学会の論文
- 1986-02-25
著者
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YAMANE TSUNEO
Laboratory of Molecular Biotechnology, Division of Molecular and Cellular Mechanisms, graduate Schoo
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Yamane Tsuneo
Laboraotory Of Bioreaction Engineering Department Of Food Science And Technology Faculty Of Agricult
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Shimizu S
Laboratory Of Bioreaction Engineering Department Of Food Science And Technology School Of Agricultur
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Shimizu Shoichi
Laboraotory Of Bioreaction Engineering Department Of Food Science And Technology Faculty Of Agricult
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Shiimizu Susumu
Department Of Food Science And Technology Faculty Of Agriculture Nagoya University
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Suzuki T
(present Address)government Industrial Research Institute Of Nagoya Ministry Of International Trade
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SUZUKI TAKAHIRO
Laboratory of Bioreaction Engineering, Department of Food Science and Technology, School of Agricult
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YASUDA TAKASHI
Laboratory of Bioreaction Engineering, Department of Food Science and Technology, School of Agricult
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Shimizu Shoichi
Daiichi Pharmaceutical Co. Ltd.
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Yamane Tsuneo
Laboratory Of Molecular Biotechnology Graduate School Of Bio- And Agro-sciences Nagoya University
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Yasuda Takashi
Laboratory Of Bioreaction Engineering Department Of Food Science And Technology School Of Agricultur
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