In Vitro Construction and Screening of a Burkholderia cepacia Lipase Library Using Single-Molecule PCR and Cell-Free Protein Synthesis(METHODS)
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概要
- 論文の詳細を見る
A mutant library of Burkholderia cepacia lipase KWI-56 was constructed on microplates by a cell-free process and tested with a chromogenic assay. This high-throughput construction system can be used to screen mutant proteins based on their catalytic activity.
- 社団法人日本生物工学会の論文
- 2002-07-25
著者
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Nakano Hideo
Laboratory of Molecular Biotechnology, Graduate School of Bioagricultural Sciences, Nagoya Universit
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YAMANE TSUNEO
Laboratory of Molecular Biotechnology, Division of Molecular and Cellular Mechanisms, graduate Schoo
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Yang Junhao
Laboratory Of Molecular Biotechnology Graduate School Of Biological & Agricultural Sciences Nago
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Koga Yuichi
Laboratory Of Molecular Biotechnology Graduate School Of Biological & Agricultural Sciences Nago
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Yamane T
Laboratory Of Molecular Biotechnology Graduate School Of Bioagricultural Sciences Nagoya University:
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Yamane Tsuneo
Laboraotory Of Bioreaction Engineering Department Of Food Science And Technology Faculty Of Agricult
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Yamane Tsuneo
Laboratory Of Bioreaction Engineering Department Of Food Science And Technology School Of Agricultur
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Kobayashi K
Department Of Chemical And Biological Sciences Faculty Of Science Japan Women's University
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Kohda K
Toyota Central R&d Laboratories Inc.
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Yamane T
Laboratory Of Molecular Biotechnology Graduate School Of Biological & Agricultural Sciences Nago
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Yamane T
Laboratory Of Bioreaction Engineering Department Of Food Science And Technology School Of Agricultur
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KOBAYASHI KOUEI
Laboratory of Molecular Biotechnology, Graduate School of Biological & Agricultural Sciences, Nagoya
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Kobayashi K
Laboratory Of Molecular Biotechnology Graduate School Of Biological & Agricultural Sciences Nago
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Kobayashi K
Shizuoka Univ. Shizuoka Jpn
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Nakano Hideo
Laboratory Of Molecular Biotechnology Graduate School Of Bioagricultural Sciences Nagoya University
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Yabuki Takashi
Protein Research Group Riken Genomic Sciences Center
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Yamane Tsuneo
Dept. Of Food Science And Technology Faculty Of Agriculture Nagoya University
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Yamane Tsuneo
Laboratory Of Molecular Biotechnology Graduate School Of Bio- And Agro-sciences Nagoya University
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Koga Yuichi
Laboratory Of Molecular Biotechnology Graduate School Of Biological & Agricultural Sciences Nago
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Nakano Hideo
Laboratory Of Molecular Biotechnology Department Of Bioengineering Sciences Graduate School Of Bioag
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Nakano Hideo
Department Of Bioengineering Sciences Graduate School Of Bioagricultural Sciences Nagoya University
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