L-Serine Production from Methanol and Glycine with an Immobilized Methylotroph
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概要
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L-Serine production from methanol and glycine was attempted using immobilized resting cells of a methylotroph, Protomonas extorquens NR 1,under automatically controlled conditions. A Ca-alginate system was selected. The conditions for L-serine formation were optimized at 30℃. A concentration of glycine 100 g・l^<-1> which was the optimum concentration for L-serine production by free resting cells was used in the reaction mixture. The optimum concentrations of methanol and dissolved oxygen were 20 g・l^<-1> and 5 ppm, respectively. Under the optimum conditions, 11.3 g・l^<-1> of L-serine was produced within 36 h. The selectivities (mole of L-serine/mole of substrate consumed) of L-serine from methanol and glycine were 4.5% and 95.1%, respectively. The size of gel beads affected the L-serine formation rate. The initial rate of L-serine formation decreased with an increase in the size of beads. However, the L-serine formation rate increased at elevated concentrations of dissolved oxygen, even with large sized beads. This result implies that the oxygen diffusion inside the gel beads limited the L-serine formation rate. The observed effectiveness factor of the immobilized cells could be estimated by the theoretical effectiveness factor of the zero-order reaction with respect to the dissolved oxygen.Repeated use was not feasible without reactivation of the immobilized cells. Reusability was examined by reactivation of the immobilized resting cells in appropriate media for 12 h. The reactivated immobilized resting cells were used again in the next cycle. By this procedure, several cycles of L-serine formation were made possible.
- 社団法人日本生物工学会の論文
- 1988-06-25
著者
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YAMANE TSUNEO
Laboratory of Molecular Biotechnology, Division of Molecular and Cellular Mechanisms, graduate Schoo
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Yamane Tsuneo
Laboraotory Of Bioreaction Engineering Department Of Food Science And Technology Faculty Of Agricult
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Yamane Tsuneo
Laboratory Of Bioreaction Engineering Department Of Food Science And Technology School Of Agricultur
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Shimizu S
Laboratory Of Bioreaction Engineering Department Of Food Science And Technology School Of Agricultur
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Shimizu Shoichi
Laboraotory Of Bioreaction Engineering Department Of Food Science And Technology Faculty Of Agricult
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Shiimizu Susumu
Department Of Food Science And Technology Faculty Of Agriculture Nagoya University
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SIRIROTE PRAMOTE
Laboratory of Bioreaction Engineering, Department of Food Science and Technology, Faculty of Agricul
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Shimizu Shoichi
Daiichi Pharmaceutical Co. Ltd.
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Yamane Tsuneo
Laboratory Of Molecular Biotechnology Graduate School Of Bio- And Agro-sciences Nagoya University
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Sirirote Pramote
Laboratory Of Bioreaction Engineering Department Of Food Science And Technology School Of Agricultur
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