Purification and Properties of the Endo-1,4-β-Glucanase from Ruminococcus albus and Its Gene Product in Escherichia coli

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概要

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• Endoglucanases, EGI and EgI, were produced from the same Ruminococcus albus gene in R. albus and recombinant Escherichia coli, respectively. EGI was purified from R. albus culture supernatant and EgI was extracted from the transformant E. coli(JM101/pURA1)and purified. The purified enzymes EGI and EgI revealed maximum endoglucanase activity at a same pH of 6.8 and a temperature of 37℃. Both enzymes were stable at temperatures below 30℃. In addition, about 10% of their orginal activities were conserved even after boiling for 10 min. Amino acid sequences of both enzymes at the N-terminal(Ala-Ala-Asp-Glu-Ser-Glu-Thr-Glu-Asn-Val-Pro-Val-Ser-Gln-Thr-His-)were consistent with each other. The antiserum against EgI reacted with both EgI and EGI, indicating that both their protein moieties were the same immunologically. However, the molecular size of EGI(43,000)was larger than that of EgI(39,000)due to the presence of sugar moiety. The specific activity(54unit/mg)of EGI was almost double that(27units/mg)of EgI. EGI was immunologically different from the endoglucanase purified in the previous paper[Ohmiya et al. : Carbohydrate Res., 166,145-155(1987)].

• 社団法人日本生物工学会の論文
• 1991-04-25

著者

• Deguchi Hiroyuki

  Department of Applied Science, Faculty of Engineering, Kyushu University

• Ohmiya Kunio

  Department Of Food Science And Technology Faculty Of Agriculture Nagoya University

• Ohmiya Kunio

  Faculty Of Bioresources Mie University

• MATSUDA Tsukasa

  Department of Food Science ande Tecknology, School of Agriculture, Nagoya University

• SHIMIZU SHOICHI

  Department of Food Science and Technology, Faculty of Agriculture, Nagoya University

• Sasaki Takuji

  Department Of Molecular Genetics National Institute Of Agrobiological Sciences

• Sasaki Takuji

  Department Of Food Science And Technology School Of Agriculture Nagoya University

• WATANABE YUICHI

  Department of Urology, Okayama Rosai Hospital

• Shimizu Shoichi

  Laboraotory Of Bioreaction Engineering Department Of Food Science And Technology Faculty Of Agricult

• Shimizu S

  Professor Emeritus Nagoya University

• Shiimizu Susumu

  Department Of Food Science And Technology Faculty Of Agriculture Nagoya University

• Deguchi Hiroyuki

  Department Of Applied Science Faculty Of Engineering Kyushu University

• Matsuda T

  Department Of Applied Molecular Biosciences School Of Bioagricultural Sciences Nagoya University

• Matsuda T

  Department Of Applied Molecular Biosciences Graduate School Of Bioagricultural Sciences Nagoya Unive

• Matsuda Tsukasa

  School Of Agriculture Nagoya University

• Matsuda Tsukasa

  Department Of Applied Biological Sciences School Of Agricultural Sciences Nagoya University

• Ohmiya Kunio

  Department Of Bioresources School Of Bioresources Mie University

• Shimizu Shoichi

  Daiichi Pharmaceutical Co. Ltd.

• Shimizu Shoichi

  Department Of Chemistry Faculty Of Science Kyoto University

• WATANABE Yuichi

  Laboratory of Bioreaction Engineering, School of Agriculture, Nagoya University

• Sasaki Takuji

  Department Of Food Science And Technology Faculty Of Agriculture Nagoya University

• Sasaki Takuji

  Natl. Inst. Agrobiological. Resources

• Watanabe Yuichi

  Laboratory Of Bioreaction Engineering School Of Agriculture Nagoya University

• Matsuda Tsukasa

  Department Of Applied Biological Science School Of Agricultural Science Nagoya University

• Deguchi Hiroyuki

  Department Of Food Science And Technology School Of Agriculture Nagoya University

• Watanabe Yuichi

  Department Of Biology Faculty Of Science Niigata University

• Watanabe Yuichi

  Department Of Anatomy School Of Medicine Tokushima University

• SHIMIZU Shoichi

  Department of Applied Chemistry, Shibaura Institute of Technology

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