Interaction between a Type-II Dockerin Domain and a Type-II Cohesin Domain from Clostridium thermocellum Cellulosome

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概要

• 論文の詳細を見る

• The interaction between the type-II dockerin domain of the scaffoldin protein CipA and the type-II cohesin domain of the outer layer protein SdbA is the fundamental mechanism for anchoring the cellulosome to the cell surface of Clostridium thermocellum. We constructed and purified a dockerin polypeptide and a cohesin polypeptide, and determined affinity constants of the interaction between them by the surface plasmon resonance method. The dissociation constant (KD) value was 1.8×10−9 M, which is a little larger than that for the combination of a type-I dockerin and a type-I cohesin.

• 社団法人 日本農芸化学会の論文
• 2004-04-23

著者

• Ohmiya K

  Graduate School Of Bioresources Mie Univ.

• Ohmiya Kunio

  Faculty Of Bioresources Mie University

• KIMURA Tetsuya

  Faculty of Bioresources, Mie University

• Kajino T

  Toyota Central Research & Development Labs. Inc.

• Kajino T

  Special Research Laboratory Ii Toyota Central R&d Laboratories Inc.

• Kajino Tsutomu

  Special Research Laboratory Ii Toyota Central R&d Laboratories Inc.

• Kimura Tetsuya

  National Center For Seeds And Seedlings

• Karita Shuichi

  Faculty of Bioresources, Mie University

• SAKKA Kazuo

  Faculty of Bioresources, Mie University

• JINDOU Sadanari

  Faculty of Bioresources, Mie University

• INAGAKI Minoru

  Faculty of Bioresources, Mie University

• BEGUIN Pierre

  Department of Biotechnology, Pasteur Institute

• Beguin Pierre

  Department Of Biotechnology Pasteur Institute

• Sakka Kazuo

  Faculty Of Bioresources Mie University

• Karita Shuichi

  Faculty Of Bioresources Mie University

• Inagaki Minoru

  Faculty Of Bioresources Mie University

• Jindou Sadanari

  Faculty Of Bioresources Mie University

• Kimura Tetsuya

  Graduate School Of Bioresources Mie University

• Kimura Tetsuya

  Faculty Of Bioresources Mie University

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