Cloning, DNA Sequencing, and Expression of the β1,4-Mannanase Gene from a Marine Bacterium, Vibrio sp. Strain MA-138
スポンサーリンク
概要
- 論文の詳細を見る
The manA gene encoding an extracellular β-1,4-mannanase of a marine bacterium, Vibrio sp. strain MA-138,was cloned and sequenced. The manA gene consists of an open reading frame of 1185 nucleotides encoding a protein of 395 amino acids with a molecular weight of 43,097. A putative ribosome-binding site and promoter region were identified in the DNA sequence. ManA of Vibrio sp. strain MA-138 was classified into family 5 of the glycosyl hydrolases and is highly homologous to the ManAs of Caldocellum saccharolyticum (sequence indentity : 53%) and Streptomyces lividans (sequence identity : 51%). The N-terminal amino acid sequence of the recombinant enzyme was completely in agreement with that deduced from the nucleotide sequence and that of the enzyme purified from strain MA-138. Although the molecular weight of the recombinant ManA was smaller than that of the enzyme from strain MA-138 on SDS-polyacrylamide gel electrophoresis, this was attributed to linkage of the carbohydrate chain to the latter protein. The enzymatic properties of the recombinant ManA was similar to those of the enzyme from strain MA-138.
- 社団法人日本生物工学会の論文
- 1997-02-25
著者
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TAMARU YUTAKA
Faculty of Bioresources, Mie University
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Ohmiya Kunio
Faculty Of Bioresources Mie University
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Araki T
Faculty Of Bioresources Mie University
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Arai Takamitsu
Faculty Of Bioresources And Center For Molecular Biology And Genetics Mie University
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KIMURA Tetsuya
Faculty of Bioresources, Mie University
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Tamaru Yutaka
Faculty Of Bioresources Mie University
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Kajino T
Special Research Laboratory Ii Toyota Central R&d Laboratories Inc.
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Tamaru Y
Mie Univ. Mie Jpn
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Kimura Tetsuya
National Center For Seeds And Seedlings
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SAKKA Kazuo
Faculty of Bioresources, Mie University
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ARAKI TOSHIYOSHI
Faculty of Bioresources, Mie University
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MORISHITA TATSUO
Faculty of Bioresources, Mie University
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Araki Toshiyoshi
Faculty Of Bioresources Mie University
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Sakka Kazuo
Faculty Of Bioresources Mie University
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Morishita Tatsuo
Faculty Of Bioresources Mie University
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Kimura Tetsuya
Faculty Of Bioresources Mie University
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