Promoter of Arabidopsis thaliana Phosphate Transporter Gene Drives Root-Specific Expression of Transgene in Rice(Plant Biotechnology)

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概要

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• The PHT1 promoter::GUS fusion gene was constructed and introduced into Arabidopsis and rice by Agrobacterium-mediated transformation. Strong β-glucuronidase (GUS) activity was detected in roots and showed phosphate starvation induction both in Arabidopsis and rice. In contrast, GUS activity in aerial tissues such as those of the leaf and stem was low. In situ GUS staining of root tissue indicated that PHT1 was expressed in root hairs and the outer layer of the main roots, but not in root tips. The PHT1 promoter has a desirable character for biotechnological transgene expression in monocot rice plants.

• 社団法人日本生物工学会の論文
• 2005-01-25

著者

• Ohmiya K

  Graduate School Of Bioresources Mie Univ.

• Ohmiya Kunio

  Faculty Of Bioresources Mie University

• KIMURA Tetsuya

  Faculty of Bioresources, Mie University

• Tomita Keiji

  Forestry Research Institute Oji Paper Co. Ltd.

• Kajino T

  Special Research Laboratory Ii Toyota Central R&d Laboratories Inc.

• Kimura Tetsuya

  National Center For Seeds And Seedlings

• SAKKA Kazuo

  Faculty of Bioresources, Mie University

• KOYAMA TAKAYOSHI

  Faculty of Bioresources, Mie University

• ONO TOSHIRO

  Faculty of Bioresources, Mie University

• SHIMIZU MASAMI

  Faculty of Bioresources, Mie University

• JINBO TETSURO

  Faculty of Bioresources, Mie University

• MIZUNO RIE

  Forestry Research Institute, Oji Paper Co. Ltd.

• MITSUKAWA NORIHIRO

  Forestry Research Institute, Oji Paper Co. Ltd.

• KAWAZU TETSU

  Forestry Research Institute, Oji Paper Co. Ltd.

• Ono T

  Shigei Medical Research Inst. Okayama

• Ohta T

  Numazu Industrial Res. Inst. Shizuoka Prefecture Numazu‐shi Jpn

• Mizuno Rie

  Forestry Research Institute Oji Paper Co. Ltd.

• Sakka Kazuo

  Faculty Of Bioresources Mie University

• Kawazu Tetsu

  Forestry Research Institute Oji Paper

• Jinbo Tetsuro

  Faculty Of Bioresources Mie University

• Mitsukawa Norihiro

  Laboratory Of Genetic Engineering Faculty Of Agriculture

• Mitsukawa Norihiro

  Mitsui Plant Biotech.res.inst.

• Kimura Tetsuya

  Graduate School Of Bioresources Mie University

• Shimizu Masami

  Faculty Of Bioresources Mie University

• Mitsukawa Norihiro

  Forestry Research Institute Oji Paper Co. Ltd.:(present Address)toyota Central R&d Labs. Inc.

• Mitsukawa Norihiro

  Mitsui Plant Biotech. Res. Inst.

• 光川 典宏

  豊田中研

• Kimura Tetsuya

  Faculty Of Bioresources Mie University

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