The Nitrate Reductase Gene from a Shoyu Koji Mold, Aspergillus oryzae KBN616

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概要

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• A niaD gene encoding nitrate reductase was isolated from Aspergillus oryzae KBN616 and sequenced. The structural gene comprises 2973bp and 868 amino acids, which showed a high degree of similarity to nitrate reductases from other filamentous fungi. The coding sequence is interrupted by six introns varying in size from 48 to 98bp. The intron positions are all conserved among the niaD genes from A. oryzae, Aspergillus nidulans, and Aspergillus niger. A homologous transformation system was developed for an industrial shoyu koji mold, A. oryzae KBN616, based on the nitrate reductase (niaD) of the nitrate assimilation pathway.

• 社団法人日本農芸化学会の論文
• 1995-09-23

著者

• Kojima Yuzo

  Department Of Bioscience Faculty Of Bioresources Center For Molecular Biology And Genetics Mie Unive

• Ohmiya Kunio

  Faculty Of Bioresources Mie University

• Kajino T

  Special Research Laboratory Ii Toyota Central R&d Laboratories Inc.

• Kitamoto Noriyuki

  Food Research Center, Aichi Industrial Technology Institute

• TSUKAGOSHI Norihiro

  Department of Food Science and Technology, Faculty of Agriculture, Nagoya University

• KITO YUKIO

  Food Research Institute, Aichi Prefectural Government

• KIMURA TETSUYA

  Food Research Institute, Aichi Prefectural Goverment

• Kitamoto N

  Food Research Center Aichi Industrial Technology Institute

• Kitamoto Noriyuki

  Food Research Center Aichi Industrial Technology Institute

• Tsukagoshi N

  Department Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya

• Tsukagoshi Norihiro

  Department Of Applied Biological Sciences Faculty Of Agriculture Nagoya University

• Kito Yukio

  Food Research Institute Aichi Prefectural Government

• Kitamoto Noriyuki

  Food Res. Center Aichi Ind. Tech. Inst.

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