Stable Expression of a Thermostable Xylanase of Clostridium thermocellum in Cultured Tobacco Cells

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概要

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• Two distinct domains of the xynA gene from Clostridium thermocellum encoding a xylanase catalytic domain (XynA1) and a xylanase catalytic domain with a cellulose binding domain (XynA2) under the control of the cauliflower mosaic virus 35S promoter were electroporated into cultured tobacco BY-2 cells. Transgenic BY-2 calli expressing xylan-hydrolyzing activity were obtained at high frequency for both genes. Western blot analysis using an anti-XynA antibody indicated that XynA1 and XynA2 were produced in these calli

• 社団法人日本生物工学会の論文
• 2003-04-25

著者

• Ohmiya K

  Graduate School Of Bioresources Mie Univ.

• Ohmiya Kunio

  Faculty Of Bioresources Mie University

• KIMURA Tetsuya

  Faculty of Bioresources, Mie University

• Kajino T

  Special Research Laboratory Ii Toyota Central R&d Laboratories Inc.

• SAKKA Kazuo

  Faculty of Bioresources, Mie University

• MIZUTANI TOMOMI

  Faculty of Bioresources,Mie University

• Sakka Kazuo

  Faculty Of Bioresources Mie University

• MIZUTANI Tomomi

  Graduate School of Bioresources, Mie University

• Takahashi Masaaki

  Mie Pref. Institute Of Public Health & Environmental Science

• Kimura Tetsuya

  Graduate School Of Bioresources Mie University

• Mizutani Takaaki

  Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University

• Kimura Tetsuya

  Faculty Of Bioresources Mie University

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