Conversion of Chitinous Wastes to Hydrogen Gas by Clostridium paraputrificum M-21

概要

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The chitinolytic bacterium Clostridium paraputrificum strain M-21 produced 2.2 and 1.5 mol hydrogen gas from 1 mol N-acetyl-D-glucosamine(GlcNAc)and ball-milled chitin equivalent to 1 mol of GlcNAc, respectively, at pH 6.0. In addition, strain M-21 efficiently degraded and fermented ball-milled raw shrimp and lobster shells to produce hydrogen gas : 11.4 mmol H_2 from 2.6 g of the former and 7.8 mmol H_2 from 1.5 g of the latter. Hydrogen evolution from these shell wastes were enhanced two fold by employing acid and alkali pretreatment. Waste from the starch industry was also converted to hydrogen. When C.paraputrificum M-21 was cultivated on ball-milled chitin and ball-milled shrimp shells for 14 and 12h, respectively, chitinases ChiA and/or ChiB were detected as the major chitinase species in the supernatant of the cultures, suggesting that they play a critical role in the degradation of chitinous materials.
公益社団法人日本生物工学会の論文
2001-04-25